Characterization of Lipid Order and Domain Formation in Model Membranes Using Fluorescence Microscopy and Spectroscopy.

Cell Membrane / metabolism Membrane Lipids / metabolism Membrane Microdomains / metabolism Microscopy, Fluorescence / methods Models, Theoretical Spectrometry, Fluorescence / methods
FRET Fluorescence anisotropy Giant unilamellar vesicles Lipid domains Model membranes Vesicle integrity

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2021
Historique:
entrez: 10 8 2020
pubmed: 10 8 2020
medline: 18 3 2021
Statut: ppublish

Résumé

Fluorescence-based techniques have been an integral factor in the study of cellular and model membranes. Fluorescence studies carried out on model membranes have provided valuable structural information and have helped reveal mechanistic detail regarding the formation and properties of ordered lipid domains, commonly known as lipid rafts. This chapter focuses on four techniques, based on fluorescence spectroscopy or microscopy, which are commonly used to analyze lipid rafts. The techniques described in this chapter may be used in a variety of ways and in combination with other techniques to provide valuable information regarding lipid order and domain formation, especially in model membranes.

Identifiants

DOI: 10.1007/978-1-0716-0814-2_15 PMID: 32770512 PMC: PMC8725914
pubmed: 32770512
doi: 10.1007/978-1-0716-0814-2_15
pmc: PMC8725914
mid: NIHMS1742734
doi:

Substances chimiques

Membrane Lipids 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

271-282

Subventions

Organisme : NIEHS NIH HHS
ID : R15 ES030140
Pays : United States

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Auteurs

Andrew Fuhrer (A)

Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, Athens, OH, USA.

Amir M Farnoud (AM)

Biomedical Engineering Program, Russ College of Engineering and Technology, Ohio University, Athens, OH, USA. farnoud@ohio.edu.
Department of Chemical and Biomolecular Engineering, Ohio University, Athens, OH, USA. farnoud@ohio.edu.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Characterization of Lipid Order and Domain...
questionsmedicales.fr › Lipides › Lipides membranaires › Characterization of Lipid Order and Domain...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Microdomaines membranaires › Characterization of Lipid Order and Domain...
questionsmedicales.fr › Techniques d'investigation › Microscopie de fluorescence › Characterization of Lipid Order and Domain...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Characterization of Lipid Order and Domain...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrométrie de fluorescence › Characterization of Lipid Order and Domain...