Detection of Functionalized Sphingolipid Analogs in Detergent-Resistant Membranes of Immune Cells.
Azido-modified ceramide
Detergent-resistant membranes (DRMs)
Fluorescence analysis
Immunoblots
Sucrose gradient
Ultracentrifugation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
10
8
2020
pubmed:
10
8
2020
medline:
18
3
2021
Statut:
ppublish
Résumé
The analysis of protein enrichment in the detergent-resistant membranes (DRMs) isolated from immune cells enables us to analyze a link between the membrane lipid dynamics and cell activation. Here, we describe the fractionation of detergent-resistant membranes and the correlative analysis of the enrichment of T cell receptor (TCR) and ω-azido-modified synthetic ceramide in those fractions upon TCR stimulation.
Identifiants
pubmed: 32770515
doi: 10.1007/978-1-0716-0814-2_18
doi:
Substances chimiques
Ceramides
0
Detergents
0
Membrane Lipids
0
Membrane Proteins
0
Receptors, Antigen, T-Cell
0
Sphingolipids
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM