Pistacia integerrima alleviated Bisphenol A induced toxicity through Ubc13/p53 signalling.
Animals
Antioxidants
/ administration & dosage
Apoptosis
/ drug effects
Apoptosis Regulatory Proteins
/ genetics
Benzhydryl Compounds
/ administration & dosage
Blood Glucose
/ drug effects
Cytochromes c
/ metabolism
Dynamins
/ genetics
Female
Hypodermoclysis
Kidney
/ cytology
Lipid Metabolism
/ drug effects
Liver
/ cytology
Male
Melatonin
/ administration & dosage
Oxidative Stress
/ drug effects
Phenols
/ administration & dosage
Pistacia
/ chemistry
Plant Extracts
/ administration & dosage
Plant Tumors
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species
/ metabolism
Signal Transduction
/ drug effects
Tumor Suppressor Protein p53
/ genetics
Ubiquitin-Conjugating Enzymes
/ genetics
Up-Regulation
Antioxidants
BPA
Environmental toxicant
Pistacia integerrima gall
Toxicity
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
24
01
2020
accepted:
02
08
2020
pubmed:
10
8
2020
medline:
26
5
2021
entrez:
10
8
2020
Statut:
ppublish
Résumé
Exposure to environmental toxicants such as Bisphenol A (BPA) has raised serious health issues globally particularly in developing countries. It is ubiquitously used in the manufacturing of canned food and feeding bottles. BPA generated reactive oxygen species can lead to several diseases including cardiotoxicity. However, the endpoints stimulated in BPA cardiotoxicity yet need to be investigated. The current study was aimed to investigate the underlying molecular pathways which may contribute in revealing the protective effects of Pistacia integerrima against BPA induced oxidative stress. The dose of 100 µg/kg BW of BPA, 200 mg/kg BW P. integerrima, and 4 mg/kg BW melatonin was administered to Sprague Dawley rats. Present results of western blotting and qRT-PCR showed the increased expression of p53, PUMA and Drp1, while downregulation of Ubc13 in heart tissues of BPA treated group whereas the levels were reversed upon treatment with P. integerrima. The role of BPA in heart tissue apoptosis was further confirmed by the increased level of P-p53, cytochrome C and disrupted cellular architecture whereas the P. integerrima has shown its ameliorative potential by mitigating the adverse effects of BPA. Moreover, the oxidant, antioxidant, lipid, and liver markers profile has also revealed the therapeutic potential of P. integerrima by maintaining the levels in the normal range. However, melatonin has also manifested the normalized expression of apoptotic markers, biochemical markers, and tissue architecture. Conclusively, the data suggest that P. integerrima may be a potential candidate for the treatment of BPA induced toxicity by neutralizing the oxidative stress through Ubc13/p53 pathway.
Identifiants
pubmed: 32770526
doi: 10.1007/s11033-020-05706-x
pii: 10.1007/s11033-020-05706-x
doi:
Substances chimiques
Antioxidants
0
Apoptosis Regulatory Proteins
0
Bbc3 protein, rat
0
Benzhydryl Compounds
0
Blood Glucose
0
Phenols
0
Plant Extracts
0
Reactive Oxygen Species
0
Tp53 protein, rat
0
Tumor Suppressor Protein p53
0
Cytochromes c
9007-43-6
Ube2n protein, rat
EC 2.3.2.23
Ubiquitin-Conjugating Enzymes
EC 2.3.2.23
Dnm1l protein, rat
EC 3.6.5.5
Dynamins
EC 3.6.5.5
Melatonin
JL5DK93RCL
bisphenol A
MLT3645I99
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6545-6559Subventions
Organisme : Higher Education Commission, Pakistan
ID : NRPU
Organisme : Quaid-i-Azam University
ID : URF
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