Fetal bovine serum-derived exosomes regulate the adipogenic differentiation of human bone marrow mesenchymal stromal cells in a cross-species manner.

Adipogenesis / genetics Animals Cell Differentiation / genetics Cell Proliferation / genetics Exosomes / drug effects Humans Mesenchymal Stem Cell Transplantation Mesenchymal Stem Cells / drug effects Osteogenesis / genetics Serum Albumin, Bovine / pharmacology
Exosomes Fetal bovine serum Human bone marrow mesenchymal stromal cells microRNA

Journal

Differentiation; research in biological diversity
ISSN: 1432-0436
Titre abrégé: Differentiation
Pays: England
ID NLM: 0401650

Informations de publication

Date de publication:
Historique:
received: 06 02 2020
revised: 15 05 2020
accepted: 21 06 2020
pubmed: 11 8 2020
medline: 14 8 2021
entrez: 11 8 2020
Statut: ppublish

Résumé

Fetal bovine serum (FBS) contains a large number of exosomes which may disturb the analysis of exosomes derived from cultured cells. We investigated the effect of FBS-derived exosomes (FBS-Exos) on the adipogenic differentiation of human bone marrow mesenchymal stromal cells (hBM-MSCs) and the underlying molecular mechanism. The uptake of FBS-Exos by hBM-MSCs could be detected by the laser confocal microscopy, and the treatment of exosomes resulted in the decreased lipid droplet formation and reduced expression of genes associated with adipogenic differentiation of hBM-MSCs. miR-1246 was identified as an abundant microRNA in FBS-Exos by public sequencing data identification and RT-qPCR validation. Moreover, miR-1246 overexpression in hBM-MSCs led to decreased adipogenic differentiation level, while miR-1246 knockdown in FBS-Exos attenuated the inhibitory effect on the adipogenic differentiation. Our results indicate that FBS-Exos inhibit the adipogenic differentiation of hBM-MSCs in a cross-species manner and miR-1246 transferred by FBS-Exos partly contributes to this effect.

Identifiants

DOI: 10.1016/j.diff.2020.06.004 PMID: 32771719
pubmed: 32771719
pii: S0301-4681(20)30038-4
doi: 10.1016/j.diff.2020.06.004
pii:
doi:

Substances chimiques

Serum Albumin, Bovine 27432CM55Q

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11-21

Informations de copyright

Copyright © 2020 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.

Auteurs

Qiongfei Zhou (Q)

Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China; National Clinical Research Center for Metabolic Diseases, Changsha, Hunan, 410011, China; Hunan Provincial Key Laboratory of Metabolic Bone Diseases, Changsha, Hunan, 410011, China.

Fen Xie (F)

Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China; National Clinical Research Center for Metabolic Diseases, Changsha, Hunan, 410011, China; Hunan Provincial Key Laboratory of Metabolic Bone Diseases, Changsha, Hunan, 410011, China; Department of Endocrinology and Metabolism, Xiangtan Central Hospital, Xiangtan, Hunan, 411100, China.

Bin Zhou (B)

Department of Spine Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China.

Chan Li (C)

Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China; National Clinical Research Center for Metabolic Diseases, Changsha, Hunan, 410011, China; Hunan Provincial Key Laboratory of Metabolic Bone Diseases, Changsha, Hunan, 410011, China.

Yijun Kang (Y)

Department of Spine Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China.

Bo Wu (B)

Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China; National Clinical Research Center for Metabolic Diseases, Changsha, Hunan, 410011, China; Hunan Provincial Key Laboratory of Metabolic Bone Diseases, Changsha, Hunan, 410011, China.

Lin Li (L)

Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China; National Clinical Research Center for Metabolic Diseases, Changsha, Hunan, 410011, China; Hunan Provincial Key Laboratory of Metabolic Bone Diseases, Changsha, Hunan, 410011, China; Department of Endocrinology and Metabolism, The First Hospital of Changsha, Changsha, Hunan, 410005, China.

Ruchun Dai (R)

Department of Metabolism and Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 410011, China; National Clinical Research Center for Metabolic Diseases, Changsha, Hunan, 410011, China; Hunan Provincial Key Laboratory of Metabolic Bone Diseases, Changsha, Hunan, 410011, China. Electronic address: dairuchun@csu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Adipogenèse › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Eucaryotes › Animaux › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Croissance › Processus de croissance cellulaire › Prolifération cellulaire › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Vésicules cytoplasmiques › Vésicules de transport › Exosomes › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Procédures de chirurgie opératoire › Transplantation › Transplantation cellulaire › Transplantation de cellules souches › Transplantation de cellules souches mésenchymateuses › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Phénomènes physiologiques de l'appareil locomoteur et du système nerveux › Phénomènes physiologiques du système locomoteur › Développement locomoteur › Biominéralisation › Développement osseux › Ostéogenèse › Fetal bovine serum-derived exosomes regulate...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines du sang › Sérumalbumine › Sérumalbumine bovine › Fetal bovine serum-derived exosomes regulate...