A 3D Cell Culture Model Identifies Wnt/
alternative splicing analysis
dedifferentiation
liver regeneration
proteomics
transcription factor motif analysis
transcriptomics
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
20
01
2020
revised:
01
06
2020
entrez:
11
8
2020
pubmed:
11
8
2020
medline:
11
8
2020
Statut:
epublish
Résumé
The liver is a highly regenerative organ. While mature hepatocytes under homeostatic conditions are largely quiescent, upon injury, they rapidly enter the cell cycle to recover the damaged tissue. In rodents, a variety of injury models have provided important insights into the molecular underpinnings that govern the proliferative activation of quiescent hepatocytes. However, little is known about the molecular mechanisms of human hepatocyte regeneration and experimental methods to expand primary human hepatocytes (PHH). Here, a 3D spheroid model of PHH is established to study hepatocyte regeneration and integrative time-lapse multi-omics analyses show that upon isolation from the native liver PHH acquire a regenerative phenotype, as seen in vivo upon partial hepatectomy. However, proliferation is limited. By analyzing global promoter motif activities, it is predicted that activation of Wnt/
Identifiants
pubmed: 32775153
doi: 10.1002/advs.202000248
pii: ADVS1906
pmc: PMC7404138
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2000248Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 The Authors. Published by WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim.
Déclaration de conflit d'intérêts
V.M.L. is founder, CEO, and shareholder of HepaPredict AB. In addition, V.M.L. discloses consultancy work for EnginZyme AB.
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