Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region.

AAV CRISPR/Cas9 HEK293T cells Lentivirus SV40 T antigen vector manufacturing

Journal

Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857

Informations de publication

Date de publication:
11 Sep 2020
Historique:
received: 10 03 2020
accepted: 06 07 2020
entrez: 11 8 2020
pubmed: 11 8 2020
medline: 11 8 2020
Statut: epublish

Résumé

The use of the human embryonic kidney (HEK) 293T cell line to manufacture vectors for

Identifiants

pubmed: 32775497
doi: 10.1016/j.omtm.2020.07.006
pii: S2329-0501(20)30155-8
pmc: PMC7397404
doi:

Types de publication

Journal Article

Langues

eng

Pagination

631-638

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Auteurs

Dahae Hailey Bae (DH)

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration (FDA), Silver Spring, MD, USA.

Michael Marino (M)

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration (FDA), Silver Spring, MD, USA.

Brian Iaffaldano (B)

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration (FDA), Silver Spring, MD, USA.

Sydney Fenstermaker (S)

Office of Biostatistics and Epidemiology, Center for Biologics Evaluation and Research, FDA, Silver Spring, MD, USA.

Sandra Afione (S)

AAV Biology Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, USA.

Takele Argaw (T)

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration (FDA), Silver Spring, MD, USA.

Jacob McCright (J)

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration (FDA), Silver Spring, MD, USA.

Anna Kwilas (A)

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration (FDA), Silver Spring, MD, USA.

John A Chiorini (JA)

AAV Biology Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, USA.

Andrew E Timmons (AE)

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration (FDA), Silver Spring, MD, USA.

Jakob Reiser (J)

Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, US Food and Drug Administration (FDA), Silver Spring, MD, USA.

Classifications MeSH