Inflammasome activation by Pseudomonas aeruginosa's ExlA pore-forming toxin is detrimental for the host.
Animals
Cytokines
/ biosynthesis
Inflammasomes
/ immunology
Inflammation
Interleukin-1alpha
/ metabolism
Interleukin-1beta
/ metabolism
Leukocidins
/ toxicity
Lung
/ immunology
Macrophages
/ immunology
Mice
Neutrophil Infiltration
Peptide Fragments
/ metabolism
Pseudomonas Infections
/ immunology
Pseudomonas aeruginosa
/ growth & development
Receptors, Interleukin-1
/ antagonists & inhibitors
Type III Secretion Systems
Virulence
microbial-cell interaction
pseudomonads
toxins
virulence
Journal
Cellular microbiology
ISSN: 1462-5822
Titre abrégé: Cell Microbiol
Pays: India
ID NLM: 100883691
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
11
07
2019
revised:
24
07
2020
accepted:
30
07
2020
pubmed:
12
8
2020
medline:
31
7
2021
entrez:
12
8
2020
Statut:
ppublish
Résumé
During acute Pseudomonas aeruginosa infection, the inflammatory response is essential for bacterial clearance. Neutrophil recruitment can be initiated following the assembly of an inflammasome within sentinel macrophages, leading to activation of caspase-1, which in turn triggers macrophage pyroptosis and IL-1β/IL-18 maturation. Inflammasome formation can be induced by a number of bacterial determinants, including Type III secretion systems (T3SSs) or pore-forming toxins, or, alternatively, by lipopolysaccharide (LPS) via caspase-11 activation. Surprisingly, previous studies indicated that a T3SS-induced inflammasome increased pathogenicity in mouse models of P. aeruginosa infection. Here, we investigated the immune reaction of mice infected with a T3SS-negative P. aeruginosa strain (IHMA879472). Virulence of this strain relies on ExlA, a secreted pore-forming toxin. IHMA879472 promoted massive neutrophil infiltration in infected lungs, owing to efficient priming of toll-like receptors, and thus enhanced the expression of inflammatory proteins including pro-IL-1β and TNF-α. However, mature-IL-1β and IL-18 were undetectable in wild-type mice, suggesting that ExlA failed to effectively activate caspase-1. Nevertheless, caspase-1/11 deficiency improved survival following infection with IHMA879472, as previously described for T3SS+ bacteria. We conclude that the detrimental effect associated with the ExlA-induced inflammasome is probably not due to hyperinflammation, rather it stems from another inflammasome-dependent process.
Substances chimiques
Cytokines
0
Inflammasomes
0
Interleukin-1alpha
0
Interleukin-1beta
0
Leukocidins
0
Peptide Fragments
0
Pseudomonas aeruginosa Cytotoxins
0
Receptors, Interleukin-1
0
Type III Secretion Systems
0
interleukin-1beta (163-171)
106021-96-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13251Informations de copyright
© 2020 John Wiley & Sons Ltd.
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