Mechanical Stress Inhibits Early Stages of Endogenous Cell Migration: A Pilot Study in an Ex Vivo Osteochondral Model.
biomaterial
cartilage
endogenous cell recruitment
hydrogel
mechanical loading
osteochondral
Journal
Polymers
ISSN: 2073-4360
Titre abrégé: Polymers (Basel)
Pays: Switzerland
ID NLM: 101545357
Informations de publication
Date de publication:
06 Aug 2020
06 Aug 2020
Historique:
received:
31
05
2020
revised:
25
07
2020
accepted:
03
08
2020
entrez:
13
8
2020
pubmed:
13
8
2020
medline:
13
8
2020
Statut:
epublish
Résumé
Cell migration has a central role in osteochondral defect repair initiation and biomaterial-mediated regeneration. New advancements to reestablish tissue function include biomaterials and factors promoting cell recruitment, differentiation and tissue integration, but little is known about responses to mechanical stimuli. In the present pilot study, we tested the influence of extrinsic forces in combination with biomaterials releasing chemoattractant signals on cell migration. We used an ex vivo mechanically stimulated osteochondral defect explant filled with fibrin/hyaluronan hydrogel, in presence or absence of platelet-derived growth factor-BB or stromal cell-derived factor 1, to assess endogenous cell recruitment into the wound site. Periodic mechanical stress at early time point negatively influenced cell infiltration compared to unloaded samples, and the implementation of chemokines to increase cell migration was not efficient to overcome this negative effect. The gene expression at 15 days of culture indicated a marked downregulation of matrix metalloproteinase
Identifiants
pubmed: 32781503
pii: polym12081754
doi: 10.3390/polym12081754
pmc: PMC7466115
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 642414
Organisme : AO Foundation
ID : 000
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