In Vitro Comparison of Passive and Active Clinical Proton Beams.
cell survival
double scattering
pencil beam scanning
proton therapy
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
06 Aug 2020
06 Aug 2020
Historique:
received:
30
06
2020
revised:
26
07
2020
accepted:
04
08
2020
entrez:
13
8
2020
pubmed:
13
8
2020
medline:
20
2
2021
Statut:
epublish
Résumé
Nowadays, the irradiation methodology in proton therapy is switching from the use of passively scattered beams to active pencil beams due to the possibility of more conformal dose distributions. The dose rates of active pencil beams are much higher than those of passive beams. The purpose of this study was to investigate whether there is any difference in the biological effectiveness of these passive and active irradiation modes. The beam qualities of double scattering and pencil beam scanning were measured dosimetrically and simulated using the Monte Carlo code. Using the medulloblastoma cell line DAOY, we performed an in vitro comparison of the two modes in two positions along the dose-deposition curve plateau and inside the Bragg peak. We followed the clonogenic cell survival, apoptosis, micronuclei, and γH2AX assays as biological endpoints. The Monte Carlo simulations did not reveal any difference between the beam qualities of the two modes. Furthermore, we did not observe any statistically significant difference between the two modes in the in vitro comparison of any of the examined biological endpoints. Our results do not show any biologically relevant differences related to the different dose rates of passive and active proton beams.
Identifiants
pubmed: 32781754
pii: ijms21165650
doi: 10.3390/ijms21165650
pmc: PMC7460593
pii:
doi:
Substances chimiques
H2AX protein, human
0
Histones
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : INSPIRE project of the European Union's Horizon 2020 Research and Innovation Programme
ID : 730983
Organisme : Czech Science Foundation
ID : 20-04109J
Organisme : German Academic Exchange Service
ID : DAAD-19-03
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