Small RNA profiles of HTLV-1 asymptomatic carriers with monoclonal and polyclonal rearrangement of the T-cell antigen receptor γ-chain using massively parallel sequencing: A pilot study.
T-cell antigen receptor
asymptomatic carriers
human T-lymphotropic virus type I
massively parallel sequencing
small RNA
Journal
Oncology letters
ISSN: 1792-1074
Titre abrégé: Oncol Lett
Pays: Greece
ID NLM: 101531236
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
19
12
2019
accepted:
21
05
2020
entrez:
13
8
2020
pubmed:
13
8
2020
medline:
13
8
2020
Statut:
ppublish
Résumé
In the present pilot study, massively parallel sequencing (MPS) technology was used to investigate cellular small RNA (sRNA) levels in the peripheral blood mononuclear cells (PBMCs) of human T-lymphotropic virus type I (HTLV-I) infected asymptomatic carriers with monoclonal (ASM) and polyclonal (ASP) T cell receptor (TCR) γ gene. Blood samples from 15 HTLV-I asymptomatic carriers (seven ASM and eight ASP) were tested for the clonal TCR-γ gene and submitted for sRNA library construction together with blood samples of five healthy controls (HCs) using Illumina sequencing platform. The sRNA-sequencing reads were aligned, annotated and profiled using various bioinformatics tools. Based on these results, possible markers were validated in the study samples by performing reverse transcription-quantitative (RT-q)PCR analysis. A total of 76 known sRNAs and 52 putative novel sRNAs were identified. Among them, 44 known and 34 potential novel sRNAs were differentially expressed in the ASM and ASP libraries compared with HCs. In addition, 10 known sRNAs were exclusively dysregulated in the ASM group and one (transfer RNA 65) was significantly upregulated in the ASP group.
Identifiants
pubmed: 32782548
doi: 10.3892/ol.2020.11803
pii: OL-0-0-11803
pmc: PMC7400997
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2311-2321Informations de copyright
Copyright: © Valadão de Souza et al.
Références
Front Microbiol. 2012 Nov 15;3:388
pubmed: 23162541
Nature. 2012 Sep 6;489(7414):101-8
pubmed: 22955620
Surgery. 2015 Oct;158(4):981-6; discussion 986-7
pubmed: 26189069
Blood. 1992 Aug 15;80(4):1012-6
pubmed: 1498321
Parasite Immunol. 2004 Nov-Dec;26(11-12):487-97
pubmed: 15771684
Hum Mol Genet. 2006 Apr 15;15 Spec No 1:R17-29
pubmed: 16651366
Blood. 1993 Oct 1;82(7):2017-24
pubmed: 8400254
Mol Aspects Med. 2010 Oct;31(5):367-82
pubmed: 20600265
J Biol Chem. 2015 Feb 27;290(9):5381-90
pubmed: 25568327
Dev Biol. 2007 Feb 1;302(1):1-12
pubmed: 16989803
Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11755-60
pubmed: 15284443
Biomed Res Int. 2017;2017:1080157
pubmed: 28529949
Am J Hematol. 1997 Apr;54(4):306-12
pubmed: 9092686
Cell Death Dis. 2012 Oct 25;3:e413
pubmed: 23096114
Nat Methods. 2014 Aug;11(8):809-15
pubmed: 24973947
Cell. 2003 Apr 4;113(1):25-36
pubmed: 12679032
J Exp Med. 2006 Oct 30;203(11):2519-27
pubmed: 17060477
FEBS Lett. 2013 Aug 2;587(15):2467-73
pubmed: 23796420
Nat Genet. 2011 Jun 05;43(7):673-8
pubmed: 21642990
J Cancer. 2017 Feb 11;8(4):578-596
pubmed: 28367238
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2999-3004
pubmed: 14973191
Pac Symp Biocomput. 2010;:80-7
pubmed: 19908360
Sci Rep. 2015 Feb 26;5:8588
pubmed: 25715874
PLoS One. 2013 Oct 08;8(10):e76450
pubmed: 24116110
PLoS One. 2014 Mar 31;9(3):e93374
pubmed: 24686382
Genes (Basel). 2010 Jun 03;1(1):70-84
pubmed: 24710011
PLoS One. 2014 Jun 16;9(6):e99442
pubmed: 24932683
Nat Commun. 2016 Jun 21;7:11702
pubmed: 27325363
Int J Cancer. 2004 Apr 20;109(4):559-67
pubmed: 14991578
Data Brief. 2016 Oct 11;9:685-688
pubmed: 27790633
Nat Rev Cancer. 2007 Apr;7(4):270-80
pubmed: 17384582
Curr Biol. 2003 Apr 29;13(9):790-5
pubmed: 12725740
Science. 2004 Dec 24;306(5705):2242-6
pubmed: 15539566
Blood. 1997 Feb 1;89(3):948-56
pubmed: 9028326
Retrovirology. 2009 Dec 17;6:117
pubmed: 20017952
Nucleic Acids Res. 2003 Jan 1;31(1):439-41
pubmed: 12520045
Biochem Biophys Res Commun. 2012 Aug 24;425(2):368-73
pubmed: 22842456
Nat Rev Genet. 2011 Nov 18;12(12):861-74
pubmed: 22094949
J Virol. 1995 May;69(5):2863-8
pubmed: 7707509
Gastroenterology. 2012 Jul;143(1):246-56.e8
pubmed: 22484120
Cancer Res. 2008 Nov 1;68(21):8976-85
pubmed: 18974142
PLoS One. 2013 Aug 28;8(8):e72662
pubmed: 24015269
Carcinogenesis. 2011 Jan;32(1):2-9
pubmed: 20952513
Cancer Metastasis Rev. 2009 Dec;28(3-4):369-78
pubmed: 20012925
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2534-7
pubmed: 6326131
J Clin Microbiol. 1992 Jun;30(6):1605-7
pubmed: 1624585
Clin Genet. 2010 Apr;77(4):306-13
pubmed: 20132241
Virology. 2008 Dec 20;382(2):257-66
pubmed: 18950829
Retrovirology. 2008 Nov 12;5:100
pubmed: 19014482
Lancet Infect Dis. 2007 Apr;7(4):266-81
pubmed: 17376384
Nature. 2005 Jun 9;435(7043):839-43
pubmed: 15944709
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415-9
pubmed: 6261256
Blood. 2008 Nov 15;112(10):4202-12
pubmed: 18713946
RNA. 2013 Apr;19(4):527-38
pubmed: 23431331
Biochim Biophys Acta. 2011 May;1812(5):592-601
pubmed: 21315819
PLoS One. 2012;7(7):e40662
pubmed: 22808228
Blood. 2010 Nov 4;116(18):3622-6
pubmed: 20656931
Hematology Am Soc Hematol Educ Program. 2000;:409-423
pubmed: 11701554
Int J Cancer. 1989 Feb 15;43(2):250-3
pubmed: 2917802
Nature. 2005 Jun 9;435(7043):834-8
pubmed: 15944708
Science. 2007 Apr 27;316(5824):575-9
pubmed: 17379774
Int J Cancer. 2012 May 15;130(10):2300-9
pubmed: 20017139
Cancer Cell. 2012 Jan 17;21(1):121-35
pubmed: 22264793
Nat Rev Genet. 2011 Feb;12(2):99-110
pubmed: 21245828
Blood. 2016 Feb 4;127(5):596-604
pubmed: 26574607
FEBS Lett. 2010 Oct 22;584(20):4313-8
pubmed: 20869963
Cell. 2003 Dec 26;115(7):787-98
pubmed: 14697198
CNS Neurosci Ther. 2013 Oct;19(10):804-12
pubmed: 23870455
Int J Hematol. 2002 Aug;76 Suppl 2:240-5
pubmed: 12430931
Development. 2008 Apr;135(7):1201-14
pubmed: 18287206
Int J Cancer. 1999 Jun 11;81(6):859-64
pubmed: 10362130
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Diagn Mol Pathol. 2004 Sep;13(3):127-34
pubmed: 15322423
Metabolism. 2015 Apr;64(4):489-97
pubmed: 25726255
Biostatistics. 2012 Apr;13(2):204-16
pubmed: 22285995
RNA. 2003 Mar;9(3):277-9
pubmed: 12592000
Nat Genet. 2003 Nov;35(3):217-8
pubmed: 14528306
Oncogene. 2003 Aug 11;22(33):5131-40
pubmed: 12910250
J Bone Miner Res. 2008 Feb;23(2):287-95
pubmed: 18197755
Int J Biochem Mol Biol. 2012;3(1):95-104
pubmed: 22509484
Blood. 2006 Dec 15;108(13):3979-82
pubmed: 16917009
Viruses. 2015 Jul 20;7(7):4047-74
pubmed: 26205403
Blood. 2009 May 14;113(20):4914-7
pubmed: 19246560
J Virol. 2014 Apr;88(7):3612-22
pubmed: 24403582
PLoS One. 2013 Oct 23;8(10):e77957
pubmed: 24194905