Ovine viperin inhibits bluetongue virus replication.

Animals Bluetongue / immunology Bluetongue virus / isolation & purification Cell Line Cloning, Molecular Gene Knockdown Techniques Immunity, Innate Iron-Sulfur Proteins / genetics Mesocricetus RNA, Messenger / metabolism Sheep, Domestic / genetics Signal Transduction / genetics Up-Regulation / immunology Virus Replication / immunology
Antiviral immunity BTV Viperin Virus replication

Journal

Molecular immunology
ISSN: 1872-9142
Titre abrégé: Mol Immunol
Pays: England
ID NLM: 7905289

Informations de publication

Date de publication:
10 2020
Historique:
received: 12 03 2020
revised: 08 07 2020
accepted: 19 07 2020
pubmed: 14 8 2020
medline: 11 11 2020
entrez: 14 8 2020
Statut: ppublish

Résumé

Viral infections can lead to interferon production, which achieves its antiviral function primarily by activating the JAK/STAT pathway and inducing multiple interferon-stimulated genes (ISGs). Although considerable ISGs have been identified in antiviral researches, little is known about ISGs in bluetongue virus (BTV) infection. Viperin is the most highly induced ISG following BTV infection, which suggests that it may play a critical role in the anti-BTV immune response. The aim of this study was to characterize ovine Viperin (oViperin) and explore whether it can inhibit BTV replication. We cloned the coding sequences (CDS) of sheep Viperin, and the sequence analysis showed that oViperin displayed a high similarity with other species. oViperin has a leucine zipper in the N-terminal, a CxxxCxxC motif in the SAM domain, and a conservative C-terminus. We found that oViperin mRNA expression was significantly up-regulated in a time- and multiplicity of infection (MOI)-dependent manner following BTV infection. oViperin overexpression resulted in a significant inhibition in BTV replication, whereas an oViperin knockdown in MDOK cells increased BTV replication. This study shows for the first time, that oViperin has antiviral activity towards BTV infection and provides important information to research the interaction between BTV and oViperin.

Identifiants

DOI: 10.1016/j.molimm.2020.07.014 PMID: 32784101
pubmed: 32784101
pii: S0161-5890(20)30429-6
doi: 10.1016/j.molimm.2020.07.014
pii:
doi:

Substances chimiques

Iron-Sulfur Proteins 0
RNA, Messenger 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

87-94

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Auteurs

Di Kang (D)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: 295484899@qq.com.

Shandian Gao (S)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: gaoshandian@caas.cn.

Zhancheng Tian (Z)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: tianzhancheng@caas.cn.

Dexuan Huang (D)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: 799408495@qq.com.

Guiquan Guan (G)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: guanguiquan@caas.cn.

Guangyuan Liu (G)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: liuguangyuan@caas.cn.

Jianxun Luo (J)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: luojianxun@caas.cn.

Junzheng Du (J)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China. Electronic address: dujunzheng@caas.cn.

Hong Yin (H)

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu, 730046, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, PR China. Electronic address: yinhong@caas.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Maladies de l'animal › Maladies des ovins › Fièvre catarrhale du mouton › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN double brin › Reoviridae › Orbivirus › Virus de la langue bleue › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-down de gènes › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Phénomènes du système immunitaire › Immunité › Immunité innée › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Métalloprotéines › Protéines de liaison au fer › Protéines à fer non hème › Ferrosulfoprotéines › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Arvicolinae › Cricetinae › Mesocricetus › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Ovis › Ovis aries › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Régulation positive › Ovine viperin inhibits bluetongue virus replication.
questionsmedicales.fr › Phénomènes microbiologiques › Phénomènes physiologiques viraux › Réplication virale › Ovine viperin inhibits bluetongue virus replication.