Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection.
Betacoronavirus
/ genetics
COVID-19
Coronavirus Infections
/ diagnosis
Coronavirus Nucleocapsid Proteins
Coronavirus RNA-Dependent RNA Polymerase
Humans
Molecular Diagnostic Techniques
/ methods
Nucleocapsid Proteins
/ genetics
Pandemics
Phosphoproteins
Pneumonia, Viral
/ diagnosis
RNA-Dependent RNA Polymerase
/ genetics
Real-Time Polymerase Chain Reaction
/ methods
Respiratory Mucosa
/ virology
SARS-CoV-2
Sensitivity and Specificity
Viral Nonstructural Proteins
/ genetics
COVID-19
RT-PCR
SARS-CoV-2
evaluation
single-tube nested
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
07 Aug 2020
07 Aug 2020
Historique:
received:
30
06
2020
revised:
28
07
2020
accepted:
05
08
2020
entrez:
14
8
2020
pubmed:
14
8
2020
medline:
21
8
2020
Statut:
epublish
Résumé
Sensitive molecular assays are critical for coronavirus disease 2019 (COVID-19) diagnosis. Here, we designed and evaluated two single-tube nested (STN) real-time RT-PCR assays, targeting SARS-CoV-2 RdRp/Hel and
Identifiants
pubmed: 32784770
pii: ijms21165674
doi: 10.3390/ijms21165674
pmc: PMC7461039
pii:
doi:
Substances chimiques
Coronavirus Nucleocapsid Proteins
0
Nucleocapsid Proteins
0
Phosphoproteins
0
Viral Nonstructural Proteins
0
nucleocapsid phosphoprotein, SARS-CoV-2
0
Coronavirus RNA-Dependent RNA Polymerase
EC 2.7.7.48
NSP12 protein, SARS-CoV-2
EC 2.7.7.48
RNA-Dependent RNA Polymerase
EC 2.7.7.48
Types de publication
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : the donations of Richard Yu and Carol Yu, the Shaw Foundation Hong Kong, May Tam Mak Mei Yin, Michael Seak-Kan Tong, Respiratory Viral Research Foundation Limited, Hui Ming, Hui Hoy and Chow Sin Lan Charity Fund Limited, Chan Yin Chuen Memorial Charitable
ID : NA
Organisme : the Consultancy Service for Enhancing Laboratory Surveillance of Emerging Infectious Diseases and Research Capability on Antimicrobial Resistance for Department of Health of the Hong Kong Special Administrative Region Government
ID : NA
Organisme : the Theme-Based Research Scheme of the Research Grants Council, Hong Kong Special Administrative Region
ID : T11/707/15
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