Biological Evaluation of Polyvinyl Alcohol Hydrogels Enriched by Hyaluronic Acid and Hydroxyapatite.
Biocompatible Materials
/ metabolism
Cell Adhesion
/ drug effects
Cell Line, Tumor
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Durapatite
/ metabolism
Humans
Hyaluronic Acid
/ metabolism
Hydrogels
/ chemistry
Materials Testing
/ methods
Osteoblasts
/ metabolism
Polyvinyl Alcohol
/ metabolism
Tissue Engineering
/ methods
Tissue Scaffolds
/ chemistry
biological evaluation
bone tissue engineering
hyaluronic acid
hydroxyapatite
polyvinyl alcohol
scaffold
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
10 Aug 2020
10 Aug 2020
Historique:
received:
20
07
2020
revised:
04
08
2020
accepted:
06
08
2020
entrez:
14
8
2020
pubmed:
14
8
2020
medline:
9
3
2021
Statut:
epublish
Résumé
This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method. We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test. The results showed that all types of scaffolds should be safe for use in the human body. The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp. This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold. Based on our results we concluded that the best PVA/HA/HAp combination is in the ratio 3:1:2.
Identifiants
pubmed: 32784986
pii: ijms21165719
doi: 10.3390/ijms21165719
pmc: PMC7461130
pii:
doi:
Substances chimiques
Biocompatible Materials
0
Hydrogels
0
polyvinyl alcohol hydrogel
0
Polyvinyl Alcohol
9002-89-5
Hyaluronic Acid
9004-61-9
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy
ID : CZ.02.1.01/0.0/0.0/16_019/0000787
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