Elevated osteogenic potential of stem cells from inflammatory dental pulp tissues by Wnt4 overexpression for treating bone defect in rats.
Inflammatory dental pulp stem cells (DPSCs-IPs)
Wnt4
bone regeneration
Journal
Annals of palliative medicine
ISSN: 2224-5839
Titre abrégé: Ann Palliat Med
Pays: China
ID NLM: 101585484
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
20
02
2020
accepted:
18
06
2020
pubmed:
14
8
2020
medline:
15
5
2021
entrez:
14
8
2020
Statut:
ppublish
Résumé
The osteogenic capacity of inflammatory dental pulp stem cells (DPSCs-IPs) is reported lower than that of normal dental pulp stem cells (DPSCs-NPs). Down-regulation of Wnt4 may be the key factor affecting the osteogenic ability of DPSCs-IPs. In order to prove that the restoration of Wnt4 expression could improve the osteogenic potential of DPSCs-IPs, Wnt4 overexpressed inflammatory dental pulp stem cells (Wnt4-DPSCs-IPs) were performed to reconstruct bone defects in rats. Human DPSCs-IPs were cultured and transfected with Wnt4 overexpression lentiviral vector. Stem cell characterization was performed by flow cytometry and induction of multidirectional differentiation. Wnt4-DPSCs-IPs were loaded onto poly (hydroxybutyrate-co-valerate) (PHBV). The compounds were engrafted into artificially-created defect in alveolar bone. The effectiveness of Wnt4-DPSCs-IPs/PHBV in bone regeneration was assessed by micro-CT and immunohistochemical staining of osteocalcin, a representative osteogenic marker. Collecting data showed that Wnt4 overexpression didn't change stem cell characteristics of DPSCs-IPs. Wnt4-DPSCs-IPs retain osteogenic, adipogenic and chondrogenic differentiation abilities. Wnt4-DPSCs-IPs/PHBV were more effective than DPSCs-IPs/PHBV in repair of rat bone defects by 3 months' post-surgical reconstruction. Restoration of Wnt4 expression could improve the osteogenic potential of DPSCs-IPs. Wnt4 restored DPSCs-IPs may be a feasible resource of seed cells for bone regeneration in future clinical application.
Sections du résumé
BACKGROUND
BACKGROUND
The osteogenic capacity of inflammatory dental pulp stem cells (DPSCs-IPs) is reported lower than that of normal dental pulp stem cells (DPSCs-NPs). Down-regulation of Wnt4 may be the key factor affecting the osteogenic ability of DPSCs-IPs. In order to prove that the restoration of Wnt4 expression could improve the osteogenic potential of DPSCs-IPs, Wnt4 overexpressed inflammatory dental pulp stem cells (Wnt4-DPSCs-IPs) were performed to reconstruct bone defects in rats.
METHODS
METHODS
Human DPSCs-IPs were cultured and transfected with Wnt4 overexpression lentiviral vector. Stem cell characterization was performed by flow cytometry and induction of multidirectional differentiation. Wnt4-DPSCs-IPs were loaded onto poly (hydroxybutyrate-co-valerate) (PHBV). The compounds were engrafted into artificially-created defect in alveolar bone. The effectiveness of Wnt4-DPSCs-IPs/PHBV in bone regeneration was assessed by micro-CT and immunohistochemical staining of osteocalcin, a representative osteogenic marker.
RESULTS
RESULTS
Collecting data showed that Wnt4 overexpression didn't change stem cell characteristics of DPSCs-IPs. Wnt4-DPSCs-IPs retain osteogenic, adipogenic and chondrogenic differentiation abilities. Wnt4-DPSCs-IPs/PHBV were more effective than DPSCs-IPs/PHBV in repair of rat bone defects by 3 months' post-surgical reconstruction.
CONCLUSIONS
CONCLUSIONS
Restoration of Wnt4 expression could improve the osteogenic potential of DPSCs-IPs. Wnt4 restored DPSCs-IPs may be a feasible resource of seed cells for bone regeneration in future clinical application.
Identifiants
pubmed: 32787349
pii: apm-20-460
doi: 10.21037/apm-20-460
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM