Clinical helminthiases in Thailand border regions show elevated prevalence levels using qPCR diagnostics combined with traditional microscopic methods.
Ancylostoma
/ isolation & purification
Ancylostomatoidea
/ isolation & purification
Animals
Ascaris lumbricoides
/ isolation & purification
Asia, Southeastern
/ epidemiology
Feces
/ parasitology
Female
Helminthiasis
/ epidemiology
Helminths
/ isolation & purification
Humans
Male
Necator americanus
/ isolation & purification
Opisthorchis
/ isolation & purification
Pathology, Molecular
Prevalence
Real-Time Polymerase Chain Reaction
Thailand
/ epidemiology
Trichuris
/ isolation & purification
Helminthiases
Kato-Katz
Southeast Asia
Thailand border regions
molecular diagnostics
qPCR
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
12 Aug 2020
12 Aug 2020
Historique:
received:
22
04
2020
accepted:
05
08
2020
entrez:
14
8
2020
pubmed:
14
8
2020
medline:
20
4
2021
Statut:
epublish
Résumé
Under-regulated national borders in Southeast Asia represent potential regions for enhanced parasitic helminth transmission and present barriers to helminthiasis disease control. Three Thailand border regions close to Myanmar, Laos and Cambodia were surveyed for clinical parasitic helminth disease. In-field microscopy was performed on stools from 567 individuals. Sub-samples were transported to Bangkok for molecular analysis comprising three multiplex qPCR assays. The overall helminth infection prevalence was 17.99% as assessed by Kato-Katz and 24.51% by qPCR. The combined prevalence of the two methods was 28.57%; the most predominant species detected were Opisthorchis viverrini (18.34%), hookworm (6.88%; Ancylostoma spp. and Necator americanus), Ascaris lumbricoides (2.29%) and Trichuris trichiura (1.76%). These data demonstrate the value of molecular diagnostics for determining more precise prevalence levels of helminthiases in Southeast Asia. Availability of such accurate prevalence information will help guide future public health initiatives and highlights the need for more rigorous surveillance and timely intervention in these regions.
Sections du résumé
BACKGROUND
BACKGROUND
Under-regulated national borders in Southeast Asia represent potential regions for enhanced parasitic helminth transmission and present barriers to helminthiasis disease control.
METHODS
METHODS
Three Thailand border regions close to Myanmar, Laos and Cambodia were surveyed for clinical parasitic helminth disease. In-field microscopy was performed on stools from 567 individuals. Sub-samples were transported to Bangkok for molecular analysis comprising three multiplex qPCR assays.
RESULTS
RESULTS
The overall helminth infection prevalence was 17.99% as assessed by Kato-Katz and 24.51% by qPCR. The combined prevalence of the two methods was 28.57%; the most predominant species detected were Opisthorchis viverrini (18.34%), hookworm (6.88%; Ancylostoma spp. and Necator americanus), Ascaris lumbricoides (2.29%) and Trichuris trichiura (1.76%).
CONCLUSIONS
CONCLUSIONS
These data demonstrate the value of molecular diagnostics for determining more precise prevalence levels of helminthiases in Southeast Asia. Availability of such accurate prevalence information will help guide future public health initiatives and highlights the need for more rigorous surveillance and timely intervention in these regions.
Identifiants
pubmed: 32787935
doi: 10.1186/s13071-020-04290-0
pii: 10.1186/s13071-020-04290-0
pmc: PMC7425172
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
416Subventions
Organisme : National Health and Medical Research Council
ID : APP1098244
Organisme : National Health and Medical Research Council
ID : ID613671
Organisme : Northern Ireland Department of Economy
ID : DFEGCRF17-18/Gobert
Organisme : National Health and Medical Research Council
ID : APP1037304
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/H019472/1
Pays : United Kingdom
Références
BMC Public Health. 2018 Sep 14;18(1):1118
pubmed: 30217180
Am J Trop Med Hyg. 2003 Jun;68(6):652-6
pubmed: 12887022
Int J Parasitol. 2015 Jun;45(7):477-83
pubmed: 25858090
PLoS Negl Trop Dis. 2016 Jan 28;10(1):e0004380
pubmed: 26820626
Exp Parasitol. 2008 Jun;119(2):217-24
pubmed: 18329641
Adv Parasitol. 2010;72:1-30
pubmed: 20624526
PLoS Negl Trop Dis. 2007 Oct 31;1(1):e75
pubmed: 17989788
Am J Trop Med Hyg. 2014 Mar;90(3):535-545
pubmed: 24445211
Trop Med Infect Dis. 2017 Oct 23;2(4):
pubmed: 30270913
Adv Parasitol. 2019;103:31-73
pubmed: 30878058
Parasitology. 2017 Jun;144(7):965-974
pubmed: 28290266
PLoS Negl Trop Dis. 2014 Sep 11;8(9):e3139
pubmed: 25211217
Am J Trop Med Hyg. 2013 Jun;88(6):1041-7
pubmed: 23509117
PLoS Negl Trop Dis. 2012;6(9):e1778
pubmed: 23029571
PLoS Negl Trop Dis. 2018 Jan 18;12(1):e0006153
pubmed: 29346440
Trop Med Int Health. 2013 May;18(5):608-14
pubmed: 23464616
Am J Trop Med Hyg. 2018 Mar;98(3):763-767
pubmed: 29363443
Am J Trop Med Hyg. 2011 Apr;84(4):594-8
pubmed: 21460016
Rev Inst Med Trop Sao Paulo. 1972 Nov-Dec;14(6):397-400
pubmed: 4675644
J Immigr Minor Health. 2016 Feb;18(1):21-7
pubmed: 25502792
PLoS Negl Trop Dis. 2020 Apr 8;14(4):e0008185
pubmed: 32267856
Adv Parasitol. 2016;91:311-97
pubmed: 27015952
PLoS Negl Trop Dis. 2016 Sep 08;10(9):e0004922
pubmed: 27607360
Korean J Parasitol. 2018 Feb;56(1):33-39
pubmed: 29529848
Vet Parasitol. 2008 Aug 1;155(1-2):67-73
pubmed: 18556131
Acta Trop. 2015 Jan;141(Pt B):150-60
pubmed: 24333545
Clin Microbiol Rev. 2015 Oct;28(4):939-67
pubmed: 26224883
Am J Trop Med Hyg. 2011 Feb;84(2):338-43
pubmed: 21292911
Parasit Vectors. 2016 Jan 27;9:31
pubmed: 26813007