The Conformational Equilibrium of the Neuropeptide Y2 Receptor in Bilayer Membranes.
NMR spectroscopy
arrestin
molecular switch
receptors
structural dynamics
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
21 12 2020
21 12 2020
Historique:
received:
27
04
2020
revised:
13
07
2020
pubmed:
14
8
2020
medline:
20
3
2021
entrez:
14
8
2020
Statut:
ppublish
Résumé
Dynamic structural transitions within the seven-transmembrane bundle represent the mechanism by which G-protein-coupled receptors convert an extracellular chemical signal into an intracellular biological function. Here, the conformational dynamics of the neuropeptide Y receptor type 2 (Y2R) during activation was investigated. The apo, full agonist-, and arrestin-bound states of Y2R were prepared by cell-free expression, functional refolding, and reconstitution into lipid membranes. To study conformational transitions between these states, all six tryptophans of Y2R were
Identifiants
pubmed: 32790043
doi: 10.1002/anie.202006075
pmc: PMC7736470
mid: NIHMS1639365
doi:
Substances chimiques
Receptors, Neuropeptide Y
0
neuropeptide Y2 receptor
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
23854-23861Subventions
Organisme : NEI NIH HHS
ID : R01 EY011500
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122491
Pays : United States
Informations de copyright
© 2020 The Authors. Published by Wiley-VCH GmbH.
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