Porcine oviductal extracellular vesicles interact with gametes and regulate sperm motility and survival.
Exosomes
Extracellular vesicles
Gametes
Oviduct
Sperm function
Journal
Theriogenology
ISSN: 1879-3231
Titre abrégé: Theriogenology
Pays: United States
ID NLM: 0421510
Informations de publication
Date de publication:
01 Oct 2020
01 Oct 2020
Historique:
received:
21
11
2019
revised:
20
05
2020
accepted:
29
05
2020
pubmed:
14
8
2020
medline:
22
6
2021
entrez:
14
8
2020
Statut:
ppublish
Résumé
Once in the female reproductive tract, spermatozoa undergo several modifications to acquire their complete fertilizing ability. Interactions between the oviductal fluid (OF) and gametes contribute to a successful fertilization. Recently, oviductal extracellular vesicles have been identified as an important part of the OF but their interactions with gametes are not fully understood. In the present study, we aim at determining the patterns of interactions between porcine oviductal extracellular vesicles (poEVs) and gametes (spermatozoa and oocytes). Moreover, we evaluate the effect of poEVs on sperm survival and motility to better understand the mechanisms by which poEVs modulate the processes leading to fertilization. Evaluation of poEVs uptake by spermatozoa showed that poEVs bind to spermatozoa in a time and dose dependent manner. Co-incubation of spermatozoa with poEVs (0.2 μg/μL) increased fresh and frozen sperm survival after 6 and 17 h, respectively. By contrast, poEVs supplementation reduced the total and progressive sperm motility after 2 h. Additionally, we demonstrated that poEVs interacted with the cumulus cells, zona pellucida (ZP) and oocyte, being able to cross the ZP. Besides, we showed that poEVs delivered their cargo into the oocyte, by the transfer of OVGP1 protein. In conclusion, our results demonstrated that poEVs are able to interact with both gametes. Besides, the findings from the present study showed that poEVs may participate in maintaining sperm viability and reducing motility, functions associated with the oviduct sperm reservoir. Although further investigations are needed, our results indicate that poEVs can be a potential tool to improve sperm life span during sperm handling and enhance IVF outcomes.
Identifiants
pubmed: 32791377
pii: S0093-691X(20)30337-X
doi: 10.1016/j.theriogenology.2020.05.043
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
240-255Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that there is no conflict of interest.