Anandamide prevents the adhesion of filamentous Candida albicans to cervical epithelial cells.
Arachidonic Acids
/ pharmacology
Biofilms
/ drug effects
Candida albicans
/ drug effects
Candidiasis
/ metabolism
Cell Adhesion
/ drug effects
Cell Line, Tumor
Cervix Uteri
/ drug effects
Endocannabinoids
/ pharmacology
Epithelial Cells
/ drug effects
Epithelium
/ drug effects
Female
Fungal Proteins
/ metabolism
Gene Expression Regulation, Fungal
/ drug effects
HeLa Cells
Humans
Hyphae
/ metabolism
Morphogenesis
/ drug effects
Polyunsaturated Alkamides
/ pharmacology
Transcription Factors
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 08 2020
13 08 2020
Historique:
received:
10
04
2020
accepted:
31
07
2020
entrez:
15
8
2020
pubmed:
15
8
2020
medline:
23
12
2020
Statut:
epublish
Résumé
Candidiasis is a fungal infection caused by Candida species that have formed a biofilm on epithelial linings of the body. The most frequently affected areas include the vagina, oral cavity and the intestine. In severe cases, the fungi penetrate the epithelium and cause systemic infections. One approach to combat candidiasis is to prevent the adhesion of the fungal hyphae to the epithelium. Here we demonstrate that the endocannabinoid anandamide (AEA) and the endocannabinoid-like N-arachidonoyl serine (AraS) strongly prevent the adherence of C. albicans hyphae to cervical epithelial cells, while the endocannabinoid 2-arachidonoylglycerol (2-AG) has only a minor inhibitory effect. In addition, we observed that both AEA and AraS prevent the yeast-hypha transition and perturb hyphal growth. Real-time PCR analysis showed that AEA represses the expression of the HWP1 and ALS3 adhesins involved in Candida adhesion to epithelial cells and the HGC1, RAS1, EFG1 and ZAP1 regulators of hyphal morphogenesis and cell adherence. On the other hand, AEA increased the expression of NRG1, a transcriptional repressor of filamentous growth. Altogether, our data show that AEA and AraS have potential anti-fungal activities by inhibiting hyphal growth and preventing hyphal adherence to epithelial cells.
Identifiants
pubmed: 32792528
doi: 10.1038/s41598-020-70650-6
pii: 10.1038/s41598-020-70650-6
pmc: PMC7426432
doi:
Substances chimiques
Arachidonic Acids
0
Endocannabinoids
0
Fungal Proteins
0
Polyunsaturated Alkamides
0
Transcription Factors
0
anandamide
UR5G69TJKH
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13728Références
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