Phosphate Transporter Profiles in Murine and Human Thymi Identify Thymocytes at Distinct Stages of Differentiation.
Animals
Biomarkers
Cell Differentiation
/ genetics
Gene Expression Profiling
Glucose Transporter Type 1
/ genetics
Humans
Immunophenotyping
Lymphocyte Subsets
/ immunology
Mice
Mice, Knockout
Phosphate Transport Proteins
/ genetics
Sodium-Phosphate Cotransporter Proteins, Type III
/ genetics
Thymocytes
/ cytology
Thymus Gland
/ cytology
Transcriptome
aging
glucose transporters
human
metabolism
mice
phosphate transporters
thymus
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2020
2020
Historique:
received:
01
04
2020
accepted:
15
06
2020
entrez:
15
8
2020
pubmed:
15
8
2020
medline:
21
4
2021
Statut:
epublish
Résumé
Thymocyte differentiation is dependent on the availability and transport of metabolites in the thymus niche. As expression of metabolite transporters is a rate-limiting step in nutrient utilization, cell surface transporter levels generally reflect the cell's metabolic state. The GLUT1 glucose transporter is upregulated on actively dividing thymocytes, identifying thymocytes with an increased metabolism. However, it is not clear whether transporters of essential elements such as phosphate are modulated during thymocyte differentiation. While PiT1 and PiT2 are both phosphate transporters in the SLC20 family, we show here that they exhibit distinct expression profiles on both murine and human thymocytes. PiT2 expression distinguishes thymocytes with high metabolic activity, identifying immature murine double negative (CD4
Identifiants
pubmed: 32793218
doi: 10.3389/fimmu.2020.01562
pmc: PMC7387685
doi:
Substances chimiques
Biomarkers
0
Glucose Transporter Type 1
0
Phosphate Transport Proteins
0
Sodium-Phosphate Cotransporter Proteins, Type III
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1562Informations de copyright
Copyright © 2020 Machado, Pouzolles, Gailhac, Fritz, Craveiro, López-Sánchez, Kondo, Pala, Bosticardo, Notarangelo, Petit, Taylor and Zimmermann.
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