Cryopreservation of Mouse Sperm for Genome Banking.


Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2021
Historique:
entrez: 16 8 2020
pubmed: 17 8 2020
medline: 16 3 2021
Statut: ppublish

Résumé

Germplasm cryobanking of transgenic rodent models is a valuable tool for protecting important genotypes from genetic drift, genetic contamination, and loss of breeding colonies due to disease or catastrophic disasters to the housing facilities as well as avoiding stress associated with domestic and international live animal shipment. Furthermore, cryopreservation of germplasm enhances management efficiencies by saving animal room space, reducing workload for staff, reducing cost of maintaining live animals, reducing the number of animals used to maintain a breeding colony, and facilitating transportation of genetics by allowing distribution of frozen germplasm rather than live animals which also reduces the risk of transfer of pathogens between facilities. Thus, effective long-term preservation methods of mouse spermatozoa are critical for future reconstitution of scientifically important mouse strains used for biomedical research.

Identifiants

pubmed: 32797423
doi: 10.1007/978-1-0716-0783-1_17
doi:

Substances chimiques

Cryoprotective Agents 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

401-412

Subventions

Organisme : NIH HHS
ID : U42 OD010918
Pays : United States

Références

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Auteurs

Yuksel Agca (Y)

College of Veterinary Medicine, University of Missouri, Columbia, MO, USA. agcay@missouri.edu.

Cansu Agca (C)

College of Veterinary Medicine, University of Missouri, Columbia, MO, USA.

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