Gelatin nanoparticles transport DNA probes for detection and imaging of telomerase and microRNA in living cells.

DNA Probes Gelatin HeLa Cells Humans MicroRNAs Nanoparticles Reproducibility of Results Telomerase / metabolism
Fluorescence imaging Molecular imprinting Telomerase microRNA

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Oct 2020
Historique:
received: 25 02 2020
revised: 22 04 2020
accepted: 27 04 2020
entrez: 17 8 2020
pubmed: 17 8 2020
medline: 15 5 2021
Statut: ppublish

Résumé

Telomerase and microRNA (miRNA) are biomarkers closely related to tumors. Simultaneous detection of both markers can improve accuracy and reliability of early diagnosis. Based on the mechanism of fluorescence resonance energy transfer (FRET), two fluorescent DNA probes were designed for telomerase and miRNA-21. The probes were wrapped by gelatin through electrostatic interaction to form nanoparticles. After that, we synthesized molecularly imprinted coating of transferrin on the surface of gelatin nanoparticles, which can avoid the immune stress response and macrophage phagocytosis to help gelatin nanoparticles enter into the cells smoothly through endocytosis. Following with the degradation of gelatin in the cells, DNA probes were released to react with telomerase and miRNA-21 and lead to the change of the fluorescence signal. Thereby the simultaneous imaging of telomerase and miRNA-21 were successfully achieved in HeLa cells and HepG2 cells. The proposed strategy shows the simultaneous imaging for different biological markers with DNA probes by preventing them from being hydrolyzed with nucleases before the determination and achieves reliable method for early diagnosis of cancer.

Identifiants

DOI: 10.1016/j.talanta.2020.121100 PMID: 32797867
pubmed: 32797867
pii: S0039-9140(20)30391-X
doi: 10.1016/j.talanta.2020.121100
pii:
doi:

Substances chimiques

DNA Probes 0
MicroRNAs 0
Gelatin 9000-70-8
Telomerase EC 2.7.7.49

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121100

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Auteurs

Yuan Zhang (Y)

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

Yida Zhang (Y)

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

Chunmeng Ma (C)

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

Guoqing Fu (G)

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

Shuai Mu (S)

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

Xiaoyan Liu (X)

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.

Haixia Zhang (H)

State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China. Electronic address: zhanghx@lzu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › Gelatin nanoparticles transport DNA probes for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Gélatine › Gelatin nanoparticles transport DNA probes for...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Gelatin nanoparticles transport DNA probes for...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Gelatin nanoparticles transport DNA probes for...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN non traduit › Petit ARN non traduit › microARN › Gelatin nanoparticles transport DNA probes for...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Gelatin nanoparticles transport DNA probes for...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Gelatin nanoparticles transport DNA probes for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Ribonucléoprotéines › Telomerase › Gelatin nanoparticles transport DNA probes for...