GPCR Genes as Activators of Surface Colonization Pathways in a Model Marine Diatom.
Genetics
Microbiology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
21 Aug 2020
21 Aug 2020
Historique:
received:
08
01
2020
revised:
25
04
2020
accepted:
28
07
2020
pubmed:
18
8
2020
medline:
18
8
2020
entrez:
18
8
2020
Statut:
ppublish
Résumé
Surface colonization allows diatoms, a dominant group of phytoplankton in oceans, to adapt to harsh marine environments while mediating biofoulings to human-made underwater facilities. The regulatory pathways underlying diatom surface colonization, which involves morphotype switching in some species, remain mostly unknown. Here, we describe the identification of 61 signaling genes, including G-protein-coupled receptors (GPCRs) and protein kinases, which are differentially regulated during surface colonization in the model diatom species, Phaeodactylum tricornutum. We show that the transformation of P. tricornutum with constructs expressing individual GPCR genes induces cells to adopt the surface colonization morphology. P. tricornutum cells transformed to express GPCR1A display 30% more resistance to UV light exposure than their non-biofouling wild-type counterparts, consistent with increased silicification of cell walls associated with the oval biofouling morphotype. Our results provide a mechanistic definition of morphological shifts during surface colonization and identify candidate target proteins for the screening of eco-friendly, anti-biofouling molecules.
Identifiants
pubmed: 32798972
pii: S2589-0042(20)30614-3
doi: 10.1016/j.isci.2020.101424
pmc: PMC7452957
pii:
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101424Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing financial interests.
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