Buckling of an Epithelium Growing under Spherical Confinement.
3D cell culture
alginate capsules
epithelial monolayer
epithelium mechanics
morphogenesis
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
14 09 2020
14 09 2020
Historique:
received:
27
02
2020
revised:
26
04
2020
accepted:
22
07
2020
pubmed:
18
8
2020
medline:
24
2
2021
entrez:
18
8
2020
Statut:
ppublish
Résumé
Many organs are formed through folding of an epithelium. This change in shape is usually attributed to tissue heterogeneities, for example, local apical contraction. In contrast, compressive stresses have been proposed to fold a homogeneous epithelium by buckling. While buckling is an appealing mechanism, demonstrating that it underlies folding requires measurement of the stress field and the material properties of the tissue, which are currently inaccessible in vivo. Here, we show that monolayers of identical cells proliferating on the inner surface of elastic spherical shells can spontaneously fold. By measuring the elastic deformation of the shell, we infer the forces acting within the monolayer and its elastic modulus. Using analytical and numerical theories linking forces to shape, we find that buckling quantitatively accounts for the shape changes of our monolayers. Our study shows that forces arising from epithelial growth in three-dimensional confinement are sufficient to drive folding by buckling.
Identifiants
pubmed: 32800097
pii: S1534-5807(20)30594-3
doi: 10.1016/j.devcel.2020.07.019
pmc: PMC7497624
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
655-668.e6Subventions
Organisme : European Research Council
ID : 311536
Pays : International
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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