Ribonuclease-Responsive DNA Nanoswitches.
Journal
Cell reports. Physical science
ISSN: 2666-3864
Titre abrégé: Cell Rep Phys Sci
Pays: United States
ID NLM: 101769239
Informations de publication
Date de publication:
22 Jul 2020
22 Jul 2020
Historique:
entrez:
18
8
2020
pubmed:
18
8
2020
medline:
18
8
2020
Statut:
ppublish
Résumé
DNA has been used in the construction of dynamic DNA devices that can reconfigure in the presence of external stimuli. These nanodevices have found uses in fields ranging from biomedical to materials science applications. Here, we report a DNA nanoswitch that can be reconfigured using ribonucleases (RNases) and explore two applications: biosensing and molecular computing. For biosensing, we show the detection of RNase H and other RNases in relevant biological fluids and temperatures, as well as inhibition by the known enzyme inhibitor kanamycin. For molecular computing, we show that RNases can be used to enable erasing, write protection, and erase-rewrite functionality for information-encoding DNA nanoswitches. The simplistic mix-and-read nature of the ribonuclease-activated DNA nanoswitches could facilitate their use in assays for identifying RNase contamination in biological samples or for the screening and characterization of RNase inhibitors.
Identifiants
pubmed: 32803173
doi: 10.1016/j.xcrp.2020.100117
pmc: PMC7425801
mid: NIHMS1615422
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM124720
Pays : United States
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