Preclinical effectiveness of an experimental tricalcium silicate cement on pulpal repair.
Odontoblast
Pulp
Tooth model
Tricalcium-silicate cement
Journal
Materials science & engineering. C, Materials for biological applications
ISSN: 1873-0191
Titre abrégé: Mater Sci Eng C Mater Biol Appl
Pays: Netherlands
ID NLM: 101484109
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
19
03
2020
revised:
18
05
2020
accepted:
05
06
2020
entrez:
19
8
2020
pubmed:
19
8
2020
medline:
27
4
2021
Statut:
ppublish
Résumé
To investigate the pulpal repair potential of an experimental zirconium-oxide containing tricalcium-silicate cement, referred to as 'TCS 50'. The effect of TCS 50 on viability, proliferation, migration, and odontoblastic differentiation of human dental pulp cells (HDPCs) was assessed using XTT assay, in-vitro wound healing assay and RT-PCR, respectively. Additionally, the pulp-capping potential was evaluated using a vital human tooth model. Statistical analysis was performed using non-parametric Kruskal-Wallis test and post-hoc test (Mann-Whitney U test). The tests were performed at a significance level of α = 0.05. The effect of TCS 50 towards HDPCs was dose dependent. Undiluted TCS 50 extract showed no immediate adverse impact on cell viability (p > .05); however, it significantly inhibited proliferation and migration of HDPCs (p < .05). A 25% diluted TCS 50 extract showed no significant effect on cell viability, proliferation or migration (p > .05), and it significantly enhanced odontoblastic differentiation of HDPCs (p < .05). In pulps capped with TCS 50 for both 2 and 4 weeks, H&E staining revealed a normal morphology of pulp tissue; mineralized foci with cellular components entrapped in the matrix were formed underneath the exposure site. Collagen I expression was weak within the matrix of mineralized foci, while the expression of nestin was positive for entrapped cellular components within the mineralized foci, indicating that the formed mineralized foci corresponded to an initial form of reparative dentin formation. TCS 50 is capable of generating an early pulp-healing reaction and therefore could serve as a promising pulp-capping agent.
Identifiants
pubmed: 32806325
pii: S0928-4931(20)31194-2
doi: 10.1016/j.msec.2020.111167
pii:
doi:
Substances chimiques
Calcium Compounds
0
Glass Ionomer Cements
0
Pulp Capping and Pulpectomy Agents
0
Silicates
0
tricalcium silicate
404G39282C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111167Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.