Effects of Diesel Exhaust Particles on Mouse Gastric Stem Cells.
cell migration
cell viability
diesel exhaust particles
oxidative stress
stem cells
Journal
Life (Basel, Switzerland)
ISSN: 2075-1729
Titre abrégé: Life (Basel)
Pays: Switzerland
ID NLM: 101580444
Informations de publication
Date de publication:
12 Aug 2020
12 Aug 2020
Historique:
received:
17
06
2020
revised:
31
07
2020
accepted:
04
08
2020
entrez:
19
8
2020
pubmed:
19
8
2020
medline:
19
8
2020
Statut:
epublish
Résumé
Stem cells have attracted many scientists because of their unique properties and therapeutic applications. However, very little is known on the environmental toxins that could affect their biological features. This study focuses on the consequences of the exposure of a cell line representative of the mouse gastric stem/progenitor (mGS) cells to diesel exhaust particles (DEPs). These immortal cells were cultured using routine protocols. The DEPs were added to the culture media at 1, 10, and 100 µg/mL for 1 to 72 h. The cells were assayed for their viability, migration, oxidative stress, and the expression of genes specific for cell proliferation, pluripotency, and death. DEPs induced a reduction in the metabolic activity of mGS cells, only at a high concentration of 100 µg/mL. However, no significant effects were detected on cell migration, oxidative stress markers (glutathione and thiobarbituric acid reactive substances), and cell death related proteins/genes. Interestingly, these findings were associated with down-regulation of Notch 2 and 3 and Bmi-1 proteins and activation of STAT3 involved in the regulation of the fate of stem cells. In conclusion, this study demonstrates that mGS cells have some resistance to oxidative stress and apoptosis when exposed to DEPs at the expense of their stemness.
Identifiants
pubmed: 32806566
pii: life10080149
doi: 10.3390/life10080149
pmc: PMC7460091
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : United Arab Emirates University
ID : 035108/15
Organisme : College of Medicine and Health Sciences, United Arab Emirates University
ID : 31M456
Organisme : College of Medicine and Health Sciences, United Arab Emirates University
ID : 31M146
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