Modelling West Nile Virus and Usutu Virus Pathogenicity in Human Neural Stem Cells.
Usutu virus
West Nile virus
Zika virus
apoptosis
flavivirus
human induced pluripotent stem cells
inflammasome
innate antiviral response
neural stem cells
virus replication
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
12 08 2020
12 08 2020
Historique:
received:
29
07
2020
accepted:
10
08
2020
entrez:
19
8
2020
pubmed:
19
8
2020
medline:
25
2
2021
Statut:
epublish
Résumé
West Nile virus (WNV) and Usutu virus (USUV) are genetically related neurotropic mosquito-borne flaviviruses, which frequently co-circulate in nature. Despite USUV seeming to be less pathogenic for humans than WNV, the clinical manifestations induced by these two viruses often overlap and may evolve to produce severe neurological complications. The aim of this study was to investigate the effects of WNV and USUV infection on human induced pluripotent stem cell-derived neural stem cells (hNSCs), as a model of the neural progenitor cells in the developing fetal brain and in adult brain. Zika virus (ZIKV), a flavivirus with known tropism for NSCs, was used as the positive control. Infection of hNSCs and viral production, effects on cell viability, apoptosis, and innate antiviral responses were compared among viruses. WNV displayed the highest replication efficiency and cytopathic effects in hNSCs, followed by USUV and then ZIKV. In these cells, both WNV and USUV induced the overexpression of innate antiviral response genes at significantly higher levels than ZIKV. Expression of interferon type I, interleukin-1β and caspase-3 was significantly more elevated in WNV- than USUV-infected hNSCs, in agreement with the higher neuropathogenicity of WNV and the ability to inhibit the interferon response pathway.
Identifiants
pubmed: 32806715
pii: v12080882
doi: 10.3390/v12080882
pmc: PMC7471976
pii:
doi:
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
Vaccine. 2016 Apr 19;34(18):2066-73
pubmed: 26993334
Euro Surveill. 2019 Jan;24(3):
pubmed: 30670139
Euro Surveill. 2009 Dec 17;14(50):
pubmed: 20070935
Viruses. 2020 Feb 08;12(2):
pubmed: 32046265
J Med Virol. 2018 Oct;90(10):1666-1668
pubmed: 29797606
J Clin Microbiol. 2010 Sep;48(9):3338-9
pubmed: 20592138
Int J Mol Sci. 2019 Oct 30;20(21):
pubmed: 31671583
Virology. 2015 Aug;482:67-71
pubmed: 25827530
J Gen Virol. 2017 Apr;98(4):662-670
pubmed: 28475031
Int J Mol Med. 2018 Apr;41(4):2263-2269
pubmed: 29344662
J Virol Methods. 2007 Dec;146(1-2):355-8
pubmed: 17604132
J Immunol. 2007 Jul 15;179(2):1166-77
pubmed: 17617610
Transbound Emerg Dis. 2019 Sep;66(5):1946-1957
pubmed: 31067011
Curr Opin Virol. 2016 Oct;20:119-128
pubmed: 27792906
Euro Surveill. 2019 May;24(21):
pubmed: 31138361
PLoS Negl Trop Dis. 2017 Sep 5;11(9):e0005913
pubmed: 28873445
Nat Commun. 2018 Jan 29;9(1):414
pubmed: 29379028
Front Endocrinol (Lausanne). 2017 Jan 20;8:4
pubmed: 28163696
Gastroenterology. 2011 Mar;140(3):1021-31
pubmed: 21111740
Emerg Infect Dis. 2016 Aug;22(8):1353-62
pubmed: 27433830
Am J Reprod Immunol. 2013 Jan;69(1):33-40
pubmed: 22967004
Clin Microbiol Infect. 2013 Oct;19(10):E451-3
pubmed: 23663225
PLoS One. 2017 Nov 16;12(11):e0188052
pubmed: 29145503
J Clin Invest. 2012 Dec;122(12):4447-60
pubmed: 23160199
Euro Surveill. 2017 Apr 6;22(14):
pubmed: 28422005
PLoS Negl Trop Dis. 2020 Apr 23;14(4):e0008223
pubmed: 32324736
Cell. 2013 Nov 7;155(4):909-921
pubmed: 24209627
Euro Surveill. 2009 Dec 17;14(50):
pubmed: 20070936
BMC Infect Dis. 2019 Oct 29;19(1):912
pubmed: 31664929
Cell Res. 2016 May;26(5):613-28
pubmed: 26915459
Virology. 2015 Nov;485:189-98
pubmed: 26280469
J Clin Virol. 2018 Oct;107:38-47
pubmed: 30176404
J Virol. 2006 Mar;80(5):2396-404
pubmed: 16474146
Euro Surveill. 2015 May 21;20(20):
pubmed: 26027485
J Infect Dis. 2013 Oct 1;208(7):1086-92
pubmed: 23821721
Endocrinology. 2010 Jun;151(6):2443-52
pubmed: 20392836
Microorganisms. 2019 Jun 26;7(7):
pubmed: 31248051
Herpesviridae. 2013 Oct 21;4(1):2
pubmed: 24144363
Stem Cell Res. 2018 Apr;28:149-152
pubmed: 29486400
J Virol. 2013 Nov;87(21):11416-25
pubmed: 23966395
Emerg Infect Dis. 2008 Aug;14(8):1232-9
pubmed: 18680646
Acta Neuropathol. 2004 Nov;108(5):453-60
pubmed: 15372281
Curr Protoc Microbiol. 2013 Nov 05;31:15D.3.1-15D.3.18
pubmed: 24510289
Emerg Infect Dis. 2013 Feb;19(2):274-7
pubmed: 23347844
Nat Immunol. 2008 Jun;9(6):641-9
pubmed: 18454151
PLoS Pathog. 2012;8(11):e1003039
pubmed: 23209411
J Immunol. 2007 Aug 1;179(3):1796-802
pubmed: 17641046
Emerg Infect Dis. 2002 Jul;8(7):652-6
pubmed: 12095429
Euro Surveill. 2019 Jul;24(28):
pubmed: 31311619
Euro Surveill. 2018 Oct;23(43):
pubmed: 30376913
J Clin Virol. 2011 Mar;50(3):221-3
pubmed: 21156352
Toxicol Sci. 2019 Mar 1;168(1):137-148
pubmed: 30452740
PLoS One. 2015 May 11;10(5):e0126277
pubmed: 25962072
J Virol. 2007 Mar;81(6):2614-23
pubmed: 17192305
J Immunol. 2014 Sep 1;193(5):2394-404
pubmed: 25070851
J Gen Virol. 2013 Apr;94(Pt 4):789-795
pubmed: 23255619
Virology. 2014 Jan 20;449:62-9
pubmed: 24418538
Sci Transl Med. 2015 Apr 22;7(284):284ra59
pubmed: 25904743
Clin Infect Dis. 2018 Apr 3;66(8):1173-1180
pubmed: 29300893
J Steroid Biochem Mol Biol. 2009 Apr;114(3-5):167-73
pubmed: 19429447
J Gen Virol. 2015 Jun;96(Pt 6):1297-1308
pubmed: 25626681
Clin Microbiol Infect. 2017 Jan;23(1):33-37
pubmed: 27677699
FEMS Microbiol Lett. 2016 Sep;363(18):
pubmed: 27549304
Euro Surveill. 2019 Nov;24(47):
pubmed: 31771697
Am J Respir Cell Mol Biol. 2004 Sep;31(3):358-64
pubmed: 15191912
Sci Rep. 2016 Jul 26;6:24530
pubmed: 27456172
Int J Mol Sci. 2017 Jan 20;18(1):
pubmed: 28117672
mBio. 2015 May 05;6(3):e00493-15
pubmed: 25944860
Virus Res. 2019 Nov;273:197753
pubmed: 31521764