Photothermogenetic inhibition of cancer stemness by near-infrared-light-activatable nanocomplexes.
Animals
Apoptosis
/ radiation effects
Blotting, Western
Calcium
/ metabolism
Calcium Channels
/ metabolism
Cell Line, Tumor
Female
Flow Cytometry
Humans
Immunohistochemistry
Infrared Rays
MCF-7 Cells
Mice
Mice, Inbred BALB C
Microscopy, Fluorescence
Nanotechnology
/ methods
Neoplastic Stem Cells
/ cytology
Reverse Transcriptase Polymerase Chain Reaction
TRPV Cation Channels
/ metabolism
Wnt Signaling Pathway
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
17 08 2020
17 08 2020
Historique:
received:
10
09
2019
accepted:
17
07
2020
entrez:
19
8
2020
pubmed:
19
8
2020
medline:
9
9
2020
Statut:
epublish
Résumé
Strategies for eradicating cancer stem cells (CSCs) are urgently required because CSCs are resistant to anticancer drugs and cause treatment failure, relapse and metastasis. Here, we show that photoactive functional nanocarbon complexes exhibit unique characteristics, such as homogeneous particle morphology, high water dispersibility, powerful photothermal conversion, rapid photoresponsivity and excellent photothermal stability. In addition, the present biologically permeable second near-infrared (NIR-II) light-induced nanocomplexes photo-thermally trigger calcium influx into target cells overexpressing the transient receptor potential vanilloid family type 2 (TRPV2). This combination of nanomaterial design and genetic engineering effectively eliminates cancer cells and suppresses stemness of cancer cells in vitro and in vivo. Finally, in molecular analyses of mechanisms, we show that inhibition of cancer stemness involves calcium-mediated dysregulation of the Wnt/β-catenin signalling pathway. The present technological concept may lead to innovative therapies to address the global issue of refractory cancers.
Identifiants
pubmed: 32807785
doi: 10.1038/s41467-020-17768-3
pii: 10.1038/s41467-020-17768-3
pmc: PMC7431860
doi:
Substances chimiques
Calcium Channels
0
TRPV Cation Channels
0
Trpv2 protein, mouse
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4117Références
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