Fluorous Soluble Cyanine Dyes for Visualizing Perfluorocarbons in Living Systems.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
16 09 2020
16 09 2020
Historique:
pubmed:
19
8
2020
medline:
16
4
2021
entrez:
19
8
2020
Statut:
ppublish
Résumé
The bioorthogonal nature of perfluorocarbons provides a unique platform for introducing dynamic nano- and microdroplets into cells and organisms. To monitor the localization and deformation of the droplets, fluorous soluble fluorophores that are compatible with standard fluorescent protein markers and applicable to cells, tissues, and small organisms are necessary. Here, we introduce fluorous cyanine dyes that represent the most red-shifted fluorous soluble fluorophores to date. We study the effect of covalently appended fluorous tags on the cyanine scaffold and evaluate the changes in photophysical properties imparted by the fluorous phase. Ultimately, we showcase the utility of the fluorous soluble pentamethine cyanine dye for tracking the localization of perfluorocarbon nanoemulsions in macrophage cells and for measurements of mechanical forces in multicellular spheroids and zebrafish embryonic tissues. These studies demonstrate that the red-shifted cyanine dyes offer spectral flexibility in multiplexed imaging experiments and enhanced precision in force measurements.
Identifiants
pubmed: 32808518
doi: 10.1021/jacs.0c07761
pmc: PMC8366720
mid: NIHMS1725325
doi:
Substances chimiques
Carbocyanines
0
Fluorescent Dyes
0
Fluorocarbons
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
16072-16081Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM135380
Pays : United States
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