Differentiation of PTH-Expressing Cells From Human Pluripotent Stem Cells.
calcium
development
hypoparathyroidism
regenerative medicine
stem cells
Journal
Endocrinology
ISSN: 1945-7170
Titre abrégé: Endocrinology
Pays: United States
ID NLM: 0375040
Informations de publication
Date de publication:
01 10 2020
01 10 2020
Historique:
received:
09
05
2020
accepted:
07
08
2020
pubmed:
19
8
2020
medline:
2
2
2021
entrez:
19
8
2020
Statut:
ppublish
Résumé
Differentiation of pluripotent stem cells into functional parathyroid-like cells would accelerate development of important therapeutic options for subjects with parathyroid-related disorders, from the design and screening of novel pharmaceutical agents to the development of durable cellular therapies. We have established a highly reproducible directed differentiation approach leading to PTH-expressing cells from human embryonic stem cells and induced pluripotent stem cells. We accomplished this through the comparison of multiple different basal media, the inclusion of the CDK inhibitor PD0332991 in both definitive endoderm and anterior foregut endoderm stages, and a 2-stage pharyngeal endoderm series. This is the first protocol to reproducibly establish PTH-expressing cells from human pluripotent stem cells and represents a first step toward the development of functional parathyroid cells with broad applicability for medicinal and scientific investigation.
Identifiants
pubmed: 32810225
pii: 5893997
doi: 10.1210/endocr/bqaa141
pmc: PMC7505176
pii:
doi:
Substances chimiques
PTH protein, human
0
Parathyroid Hormone
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : R21 CA240660
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001863
Pays : United States
Informations de copyright
© Endocrine Society 2020. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
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