Interaction of the pioneer transcription factor GATA3 with nucleosomes.
Amino Acid Motifs
/ genetics
Binding Sites
Chromatin Assembly and Disassembly
/ genetics
Chromatin Immunoprecipitation Sequencing
Cryoelectron Microscopy
Enhancer Elements, Genetic
GATA3 Transcription Factor
/ chemistry
Histones
/ metabolism
Humans
Nucleosomes
/ chemistry
Protein Binding
Zinc Fingers
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 08 2020
18 08 2020
Historique:
received:
07
09
2019
accepted:
22
07
2020
entrez:
20
8
2020
pubmed:
20
8
2020
medline:
17
9
2020
Statut:
epublish
Résumé
During cellular reprogramming, the pioneer transcription factor GATA3 binds chromatin, and in a context-dependent manner directs local chromatin remodeling and enhancer formation. Here, we use high-resolution nucleosome mapping in human cells to explore the impact of the position of GATA motifs on the surface of nucleosomes on productive enhancer formation, finding productivity correlates with binding sites located near the nucleosomal dyad axis. Biochemical experiments with model nucleosomes demonstrate sufficiently stable transcription factor-nucleosome interaction to empower cryo-electron microscopy structure determination of the complex at 3.15 Å resolution. The GATA3 zinc fingers efficiently bind their target 5'-GAT-3' sequences in the nucleosome when they are located in solvent accessible, consecutive major grooves without significant changes in nucleosome structure. Analysis of genomic loci bound by GATA3 during reprogramming suggests a correlation of recognition motif sequence and spacing that may distinguish productivity of new enhancer formation.
Identifiants
pubmed: 32811816
doi: 10.1038/s41467-020-17959-y
pii: 10.1038/s41467-020-17959-y
pmc: PMC7434886
doi:
Substances chimiques
GATA3 Transcription Factor
0
GATA3 protein, human
0
Histones
0
Nucleosomes
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4136Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM104360
Pays : United States
Organisme : Intramural NIH HHS
ID : Z01 ES101965
Pays : United States
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