Estimation of the number of synapses in the hippocampus and brain-wide by volume electron microscopy and genetic labeling.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
19 08 2020
19 08 2020
Historique:
received:
07
05
2020
accepted:
27
07
2020
entrez:
21
8
2020
pubmed:
21
8
2020
medline:
13
1
2021
Statut:
epublish
Résumé
Determining the number of synapses that are present in different brain regions is crucial to understand brain connectivity as a whole. Membrane-associated guanylate kinases (MAGUKs) are a family of scaffolding proteins that are expressed in excitatory glutamatergic synapses. We used genetic labeling of two of these proteins (PSD95 and SAP102), and Spinning Disc confocal Microscopy (SDM), to estimate the number of fluorescent puncta in the CA1 area of the hippocampus. We also used FIB-SEM, a three-dimensional electron microscopy technique, to calculate the actual numbers of synapses in the same area. We then estimated the ratio between the three-dimensional densities obtained with FIB-SEM (synapses/µm
Identifiants
pubmed: 32814795
doi: 10.1038/s41598-020-70859-5
pii: 10.1038/s41598-020-70859-5
pmc: PMC7438319
doi:
Substances chimiques
Disks Large Homolog 4 Protein
0
Dlg4 protein, mouse
0
Membrane Proteins
0
Dlgh3 protein, mouse
EC 2.7.4.8
Guanylate Kinases
EC 2.7.4.8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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