Immune-evasive human islet-like organoids ameliorate diabetes.


Journal

Nature
ISSN: 1476-4687
Titre abrégé: Nature
Pays: England
ID NLM: 0410462

Informations de publication

Date de publication:
10 2020
Historique:
received: 17 12 2018
accepted: 18 05 2020
pubmed: 21 8 2020
medline: 20 1 2021
entrez: 21 8 2020
Statut: ppublish

Résumé

Islets derived from stem cells hold promise as a therapy for insulin-dependent diabetes, but there remain challenges towards achieving this goal

Identifiants

pubmed: 32814902
doi: 10.1038/s41586-020-2631-z
pii: 10.1038/s41586-020-2631-z
pmc: PMC7872080
mid: NIHMS1595843
doi:

Substances chimiques

B7-H1 Antigen 0
CD274 protein, human 0
WNT4 protein, human 0
Wnt4 Protein 0
Glucose IY9XDZ35W2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

606-611

Subventions

Organisme : NIDDK NIH HHS
ID : K01 DK120808
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK063491
Pays : United States
Organisme : NIH HHS
ID : S10 OD023689
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA014195
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIEHS NIH HHS
ID : P42 ES010337
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK098085
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK120480
Pays : United States

Commentaires et corrections

Type : CommentIn
Type : CommentIn
Type : ErratumIn

Références

Yoshihara, E. et al. ERRγ is required for the metabolic maturation of therapeutically functional glucose-responsive β cells. Cell Metab. 23, 622–634 (2016).
pubmed: 27076077 pmcid: 4832237
Hrvatin, S. et al. Differentiated human stem cells resemble fetal, not adult, β cells. Proc. Natl Acad. Sci. USA 111, 3038–3043 (2014).
pubmed: 24516164
Rezania, A. et al. Reversal of diabetes with insulin-producing cells derived in vitro from human pluripotent stem cells. Nat. Biotechnol. 32, 1121–1133 (2014).
pubmed: 25211370
Pagliuca, F. W. et al. Generation of functional human pancreatic β cells in vitro. Cell 159, 428–439 (2014).
pubmed: 25303535 pmcid: 4617632
Kieffer, T. J. Closing in on mass production of mature human beta cells. Cell Stem Cell 18, 699–702 (2016).
pubmed: 27257758
Liu, J. S. & Hebrok, M. All mixed up: defining roles for β-cell subtypes in mature islets. Genes Dev. 31, 228–240 (2017).
pubmed: 28270515 pmcid: 5358720
Takebe, T. et al. Vascularized and functional human liver from an iPSC-derived organ bud transplant. Nature 499, 481–484 (2013).
pubmed: 23823721
Asai, A. et al. Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells. Development 144, 1056–1064 (2017).
pubmed: 28275009 pmcid: 5358109
Bader, E. et al. Identification of proliferative and mature β-cells in the islets of Langerhans. Nature 535, 430–434 (2016).
pubmed: 27398620
van der Meulen, T. et al. Urocortin3 mediates somatostatin-dependent negative feedback control of insulin secretion. Nat. Med. 21, 769–776 (2015).
pubmed: 26076035 pmcid: 4496282
Blum, B. et al. Functional beta-cell maturation is marked by an increased glucose threshold and by expression of urocortin 3. Nat. Biotechnol. 30, 261–264 (2012).
pubmed: 22371083 pmcid: 4617627
van der Meulen, T. et al. Urocortin 3 marks mature human primary and embryonic stem cell-derived pancreatic alpha and beta cells. PLoS ONE 7, e52181 (2012).
pubmed: 23251699 pmcid: 3522648
Prentki, M., Matschinsky, F. M. & Madiraju, S. R. Metabolic signaling in fuel-induced insulin secretion. Cell Metab. 18, 162–185 (2013).
pubmed: 23791483
Huang, S. M. et al. Tankyrase inhibition stabilizes axin and antagonizes Wnt signalling. Nature 461, 614–620 (2009).
pubmed: 19759537 pmcid: 19759537
Baas, M. et al. TGFβ-dependent expression of PD-1 and PD-L1 controls CD8
pubmed: 26824266 pmcid: 4749558
Martinov, T., Spanier, J. A., Pauken, K. E. & Fife, B. T. PD-1 pathway-mediated regulation of islet-specific CD4
pubmed: 27656680
Keir, M. E. et al. Tissue expression of PD-L1 mediates peripheral T cell tolerance. J. Exp. Med. 203, 883–895 (2006).
pubmed: 16606670 pmcid: 2118286
Ansari, M. J. et al. The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice. J. Exp. Med. 198, 63–69 (2003).
pubmed: 12847137 pmcid: 2196083
Ma, D. et al. PD-L1 deficiency within islets reduces allograft survival in mice. PLoS ONE 11, e0152087 (2016).
pubmed: 26990974 pmcid: 4798758
Rui, J. et al. β Cells that resist immunological attack develop during progression of autoimmune diabetes in NOD Mice. Cell Metab. 25, 727–738 (2017).
pubmed: 28190773 pmcid: 5342930
Wang, C. J. et al. Protective role of programmed death 1 ligand 1 (PD-L1) in nonobese diabetic mice: the paradox in transgenic models. Diabetes 57, 1861–1869 (2008).
pubmed: 18420489 pmcid: 2453619
Colli, M. L. et al. PDL1 is expressed in the islets of people with type 1 diabetes and is up-regulated by interferons-α and-γ via IRF1 induction. eBioMedicine 36, 367–375 (2018).
pubmed: 30269996 pmcid: 6197434
Osum, K. C. et al. Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes. Sci. Rep. 8, 8295 (2018).
pubmed: 29844327 pmcid: 5974126
Eizirik, D. L. & Mandrup-Poulsen, T. A choice of death—the signal-transduction of immune-mediated beta-cell apoptosis. Diabetologia 44, 2115–2133 (2001).
pubmed: 11793013
Russ, H. A. et al. Controlled induction of human pancreatic progenitors produces functional beta-like cells in vitro. EMBO J. 34, 1759–1772 (2015).
pubmed: 25908839 pmcid: 4516429
Nair, G. G. et al. Recapitulating endocrine cell clustering in culture promotes maturation of human stem-cell-derived β cells. Nat. Cell Biol. 21, 263–274 (2019).
pubmed: 30710150 pmcid: 6746427
Sneddon, J. B. et al. Stem cell therapies for treating diabetes: progress and remaining challenges. Cell Stem Cell 22, 810–823 (2018).
pubmed: 29859172 pmcid: 6007036
Zhou, Q. & Melton, D. A. Pancreas regeneration. Nature 557, 351–358 (2018).
pubmed: 29769672 pmcid: 6168194
Turner, M. et al. Toward the development of a global induced pluripotent stem cell library. Cell Stem Cell 13, 382–384 (2013).
pubmed: 24094319
Morizane, A. et al. MHC matching improves engraftment of iPSC-derived neurons in non-human primates. Nat. Commun. 8, 385 (2017).
pubmed: 28855509 pmcid: 5577234
Wei, Z. et al. Vitamin D switches BAF complexes to protect β cells. Nat. Commun. 173, 1135–1149 (2018).
Yoshihara, E. et al. Disruption of TBP-2 ameliorates insulin sensitivity and secretion without affecting obesity. Nat. Commun. 1, 127 (2010).
pubmed: 21119640 pmcid: 3060604
Buenrostro, J. D., Wu, B., Chang, H. Y. & Greenleaf, W. J. ATAC-seq: a method for assaying chromatin accessibility genome-wide. Curr. Protoc. Mol. Biol. 109, 21.29.1–21.29.9 (2015).
Heinz, S. et al. Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities. Mol. Cell 38, 576–589 (2010).
pubmed: 20513432 pmcid: 20513432
Dobin, A. et al. STAR: ultrafast universal RNA-seq aligner. Bioinformatics 29, 15–21 (2013).
Trapnell, C. et al. Differential analysis of gene regulation at transcript resolution with RNA-seq. Nat. Biotechnol. 31, 46–53 (2013).
pubmed: 23222703
Roberts, A., Pimentel, H., Trapnell, C. & Pachter, L. Identification of novel transcripts in annotated genomes using RNA-seq. Bioinformatics 27, 2325–2329 (2011).
pubmed: 21697122
van Dijk, D. et al. Recovering gene interactions from single-cell data using data diffusion. Cell 174, 716–729 (2018).
pubmed: 29961576 pmcid: 6771278
Macosko, E. Z. et al. Highly parallel genome-wide expression profiling of individual cells using nanoliter droplets. Cell 161, 1202–1214 (2015).
pubmed: 26000488 pmcid: 4481139
Butler, A., Hoffman, P., Smibert, P., Papalexi, E. & Satija, R. Integrating single-cell transcriptomic data across different conditions, technologies, and species. Nat. Biotechnol. 36, 411–420 (2018).
pubmed: 29608179 pmcid: 29608179
Huang da. W. et al. Extracting biological meaning from large gene lists with DAVID. Curr. Protoc. Bioinformatics Ch. 13, https://doi.org/10.1002/0471250953.bi1311s27 (2009).
Walter, W., Sánchez-Cabo, F. & Ricote, M. GOplot: an R package for visually combining expression data with functional analysis. Bioinformatics 31, 2912–2914 (2015).

Auteurs

Eiji Yoshihara (E)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
The Lundquist Institute for Biomedical Innovation, Harbor-UCLA Medical Center, Torrance, CA, USA.
David Geffen School of Medicine at UCLA, Los Angeles, USA.

Carolyn O'Connor (C)

Flow Cytometry Core Facility, Salk Institute for Biological Studies, La Jolla, CA, USA.

Emanuel Gasser (E)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Zong Wei (Z)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.
Department of Physiology and Biomedical Engineering, Mayo Clinic, Scottsdale, AZ, USA.

Tae Gyu Oh (TG)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Tiffany W Tseng (TW)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Dan Wang (D)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Fritz Cayabyab (F)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Yang Dai (Y)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Ruth T Yu (RT)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Christopher Liddle (C)

Storr Liver Centre, Westmead Institute for Medical Research and Sydney Medical School, University of Sydney, Westmead, New South Wales, Australia.

Annette R Atkins (AR)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Michael Downes (M)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA.

Ronald M Evans (RM)

Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA, USA. evans@salk.edu.
Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA, USA. evans@salk.edu.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH