Cepheid Xpert
Allplex™ RP1
Xpert® Flu/RSV XC
diagnostic agreement
molecular assays
Journal
Influenza and other respiratory viruses
ISSN: 1750-2659
Titre abrégé: Influenza Other Respir Viruses
Pays: England
ID NLM: 101304007
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
16
02
2020
revised:
22
07
2020
accepted:
27
07
2020
pubmed:
21
8
2020
medline:
26
11
2021
entrez:
21
8
2020
Statut:
ppublish
Résumé
Molecular assays based on reverse transcription-polymerase chain reaction (RT-PCR) provide reliable results for the detection of respiratory pathogens, although diagnostic agreement varies. This study determined the agreement between the RT-PCR assays (Xpert We retrospectively identified 914 patient encounters where testing with both Xpert The positive percent agreement was 95.1% for influenza A, 87.5% for influenza B, and 77.8% for RSV. The negative percent agreement was 99.4% for influenza A, 99.9% for influenza B, and 100% for RSV. The prevalence and bias-adjusted kappa was 0.98 for influenza A, 0.99 for influenza B, and 0.97 for RSV. In a sensitivity analysis, the positive percent agreement values were significantly higher during the non-influenza season than the influenza season for influenza B and RSV. The Xpert
Sections du résumé
BACKGROUND
Molecular assays based on reverse transcription-polymerase chain reaction (RT-PCR) provide reliable results for the detection of respiratory pathogens, although diagnostic agreement varies. This study determined the agreement between the RT-PCR assays (Xpert
METHODS
We retrospectively identified 914 patient encounters where testing with both Xpert
RESULTS
The positive percent agreement was 95.1% for influenza A, 87.5% for influenza B, and 77.8% for RSV. The negative percent agreement was 99.4% for influenza A, 99.9% for influenza B, and 100% for RSV. The prevalence and bias-adjusted kappa was 0.98 for influenza A, 0.99 for influenza B, and 0.97 for RSV. In a sensitivity analysis, the positive percent agreement values were significantly higher during the non-influenza season than the influenza season for influenza B and RSV.
CONCLUSIONS
The Xpert
Identifiants
pubmed: 32815622
doi: 10.1111/irv.12799
pmc: PMC7461471
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
245-253Informations de copyright
© 2020 The Authors. Influenza and Other Respiratory Viruses published by John Wiley & Sons Ltd.
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