Effects of Prepubertal Exposure to Aroclor-1221 on Reproductive Development and Transcriptional Gene Expression in Female Rats.
Age Factors
Animals
Anti-Mullerian Hormone
/ genetics
Apoptosis
/ drug effects
Aroclors
/ toxicity
Environmental Pollutants
/ toxicity
Estradiol
/ blood
Estrous Cycle
/ blood
Female
Fertilization in Vitro
Gonadotropin-Releasing Hormone
/ genetics
Hypothalamus
/ drug effects
Kisspeptins
/ genetics
Ovarian Follicle
/ drug effects
Ovary
/ drug effects
Progesterone
/ blood
Proto-Oncogene Proteins c-myc
/ genetics
Rats, Sprague-Dawley
Reproduction
/ drug effects
Sexual Development
/ drug effects
Transcription, Genetic
/ drug effects
Genetic regulation
Polychlorinated biphenyls
Prepubertal
Reproductive development
Transcriptional gene expression
Journal
Reproductive sciences (Thousand Oaks, Calif.)
ISSN: 1933-7205
Titre abrégé: Reprod Sci
Pays: United States
ID NLM: 101291249
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
05
03
2020
accepted:
04
08
2020
pubmed:
21
8
2020
medline:
24
11
2021
entrez:
21
8
2020
Statut:
ppublish
Résumé
Polychlorinated biphenyls (PCBs), as persistent organic pollutants, are environmental endocrine-disrupting chemicals (EDCs). We aim to investigate the effects of prepubertal exposure to PCBs on the reproductive development and expression and regulation of related genes in rats. Female rats were treated with Aroclor-1221 (A-1221) (4 mg/kg/day, 0.4 mg/kg/day) or castor oil daily from postnatal day (PND) 28 for 2 weeks by gavage. Morphological, histological, hormonal, and biochemical parameters were studied. Lower weight and relative weight of hypothalamus, earlier puberty onset, a longer length of the estrous cycle, lower serum estradiol and progesterone levels, accelerated ovarian folliculogenesis, and higher apoptotic index in the ovary were found. The in vitro fertilization study showed a lower fertilization rate and cleavage rate. The genetic study revealed higher expression of Kiss-1 mRNA and lower expression of GnRH mRNA in the hypothalamus and higher expression of AMH mRNA and lower expression of C-myc mRNA in the ovary. These confirmed the reproductive damage of A-1221 in rats.
Identifiants
pubmed: 32816245
doi: 10.1007/s43032-020-00290-8
pii: 10.1007/s43032-020-00290-8
doi:
Substances chimiques
Aroclors
0
Environmental Pollutants
0
Kiss1 protein, rat
0
Kisspeptins
0
Proto-Oncogene Proteins c-myc
0
aroclor 1221
11104-28-2
Gonadotropin-Releasing Hormone
33515-09-2
Progesterone
4G7DS2Q64Y
Estradiol
4TI98Z838E
Anti-Mullerian Hormone
80497-65-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
393-405Références
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