Development of a bead-based assay for detection and differentiation of field strains and four vaccine strains of type 2 porcine reproductive and respiratory syndrome virus (PRRSV-2) in the USA.
Luminex
PRRSV
detection
differentiation
field strains
vaccine strains
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
revised:
14
08
2020
received:
29
06
2020
accepted:
17
08
2020
pubmed:
21
8
2020
medline:
25
6
2021
entrez:
21
8
2020
Statut:
ppublish
Résumé
Porcine reproductive and respiratory syndrome (PRRS) remains one of the most economically devastating diseases in swine population in the United States of America. Due to high mutation rate of the PRRS virus (PRRSV) genome, it is difficult to develop an accurate diagnostic assay with high strain coverage. Differentiation of field strains from the four vaccines that have been used in the USA, namely Ingelvac PRRS MLV, Ingelvac ATP, Fostera PRRS and Prime Pac PRRS, adds an additional challenge. It is difficult to use current real-time PCR systems to detect and differentiate the field strains from the vaccine strains. Luminex xTAG technology allows us to detect more molecular targets in a single reaction with a cost similar to a single real-time PCR reaction. By analysing all available 678 type 2 PRRSV (PRRSV-2) complete genome sequences, including the 4 vaccine strains, two pairs of detection primers were designed targeting the conserved regions of ORF4-ORF7, with strain coverage of 98.8% (670/678) based on in silico analysis. The virus strains sharing ≥98% identity of the complete genomes with the vaccine strains were considered vaccine or vaccine-like strains. One pair of primers for each vaccine strain were designed targeting the nsp2 region. In silico analysis showed the assay matched 94.7% (54/57) of Ingelvac PRRS
Identifiants
pubmed: 32816334
doi: 10.1111/tbed.13808
pmc: PMC8895411
mid: NIHMS1783617
doi:
Substances chimiques
Vaccines, Attenuated
0
Viral Vaccines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1414-1423Subventions
Organisme : NIGMS NIH HHS
ID : P20 GM103418
Pays : United States
Organisme : National Pork Board
ID : 16-196
Informations de copyright
© 2020 Wiley-VCH GmbH.
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