HIV-1 Gag protein with or without p6 specifically dimerizes on the viral RNA packaging signal.
Gag
Gag p6 domain
HIV
MS
RNA
RNA binding protein
RNA-protein interaction
genomic RNA packaging
human immunodeficiency virus
mass spectrometry
native mass spectrometry
virus assembly
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
16 10 2020
16 10 2020
Historique:
received:
17
06
2020
revised:
10
08
2020
pubmed:
21
8
2020
medline:
24
2
2021
entrez:
21
8
2020
Statut:
ppublish
Résumé
The HIV-1 Gag protein is responsible for genomic RNA (gRNA) packaging and immature viral particle assembly. Although the presence of gRNA in virions is required for viral infectivity, in its absence, Gag can assemble around cellular RNAs and form particles resembling gRNA-containing particles. When gRNA is expressed, it is selectively packaged despite the presence of excess host RNA, but how it is selectively packaged is not understood. Specific recognition of a gRNA packaging signal (Psi) has been proposed to stimulate the efficient nucleation of viral assembly. However, the heterogeneity of Gag-RNA interactions renders capturing this transient nucleation complex using traditional structural biology approaches challenging. Here, we used native MS to investigate RNA binding of wild-type (WT) Gag and Gag lacking the p6 domain (GagΔp6). Both proteins bind to Psi RNA primarily as dimers, but to a control RNA primarily as monomers. The dimeric complexes on Psi RNA require an intact dimer interface within Gag. GagΔp6 binds to Psi RNA with high specificity
Identifiants
pubmed: 32817318
pii: S0021-9258(17)49781-X
doi: 10.1074/jbc.RA120.014835
pmc: PMC7573273
doi:
Substances chimiques
RNA, Viral
0
gag Gene Products, Human Immunodeficiency Virus
0
p6 gag protein, Human immunodeficiency virus 1
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
14391-14401Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM118291
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM065056
Pays : United States
Organisme : NIAID NIH HHS
ID : U54 AI150472
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI153216
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM128577
Pays : United States
Déclaration de conflit d'intérêts
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
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