Illuminating the allosteric modulation of the calcium-sensing receptor.


Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
01 09 2020
Historique:
pubmed: 21 8 2020
medline: 30 10 2020
entrez: 21 8 2020
Statut: ppublish

Résumé

Many membrane receptors are regulated by nutrients. However, how these nutrients control a single receptor remains unknown, even in the case of the well-studied calcium-sensing receptor CaSR, which is regulated by multiple factors, including ions and amino acids. Here, we developed an innovative cell-free Förster resonance energy transfer (FRET)-based conformational CaSR biosensor to clarify the main conformational changes associated with activation. By allowing a perfect control of ambient nutrients, this assay revealed that Ca

Identifiants

pubmed: 32817431
pii: 1922231117
doi: 10.1073/pnas.1922231117
pmc: PMC7474691
doi:

Substances chimiques

Ligands 0
Receptors, Calcium-Sensing 0
Receptors, G-Protein-Coupled 0
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

21711-21722

Déclaration de conflit d'intérêts

The authors declare no competing interest.

Références

Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2660-5
pubmed: 11867751
FASEB J. 2015 Oct;29(10):4174-88
pubmed: 26116702
J Pharmacol Exp Ther. 2013 Aug;346(2):229-40
pubmed: 23674604
Nat Rev Endocrinol. 2018 Dec;15(1):33-51
pubmed: 30443043
Nature. 2000 Oct 26;407(6807):971-7
pubmed: 11069170
Int J Mol Sci. 2017 Mar 21;18(3):
pubmed: 28335551
Nat Struct Mol Biol. 2004 Aug;11(8):706-13
pubmed: 15235591
Cell Metab. 2017 Apr 4;25(4):777-796
pubmed: 28380372
Nat Commun. 2019 Jun 24;10(1):2765
pubmed: 31235691
FASEB J. 2011 Jan;25(1):66-77
pubmed: 20826542
J Biol Chem. 2001 Feb 16;276(7):5316-22
pubmed: 11069904
Cell Res. 2016 May;26(5):574-92
pubmed: 27002221
Nat Chem Biol. 2015 Feb;11(2):134-40
pubmed: 25503927
J Biol Chem. 2004 Dec 10;279(50):51739-44
pubmed: 15579475
Pharmacol Rev. 2020 Jul;72(3):558-604
pubmed: 32467152
Br J Pharmacol. 2007 Mar;150(6):798-807
pubmed: 17245368
Am J Physiol. 1997 Oct;273(4):C1315-23
pubmed: 9357776
Curr Opin Pharmacol. 2015 Feb;20:95-101
pubmed: 25529199
Trends Pharmacol Sci. 2000 Oct;21(10):401-7
pubmed: 11050321
Cell Signal. 2014 May;26(5):833-48
pubmed: 24440667
Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15480-5
pubmed: 21896740
J Biol Chem. 2014 Nov 28;289(48):33529-42
pubmed: 25305020
Nat Commun. 2017 May 23;8:15530
pubmed: 28534491
Neuron. 2016 Oct 5;92(1):143-159
pubmed: 27641494
Proc Natl Acad Sci U S A. 2013 Apr 9;110(15):E1416-25
pubmed: 23487753
Nat Chem Biol. 2017 Apr;13(4):372-380
pubmed: 28135236
Nat Protoc. 2014 Jan;9(1):156-70
pubmed: 24356774
Trends Endocrinol Metab. 2003 Aug;14(6):282-8
pubmed: 12890593
Cell Res. 2020 Jul;30(7):564-573
pubmed: 32494023
J Biol Chem. 2010 Sep 24;285(39):30294-303
pubmed: 20630861
J Biol Chem. 2007 Jun 29;282(26):19000-10
pubmed: 17478419
Nature. 2020 Aug;584(7820):298-303
pubmed: 32555460
Biochemistry. 2009 Jan 20;48(2):388-98
pubmed: 19102677
J Biol Chem. 1999 Sep 24;274(39):27642-50
pubmed: 10488104
Mol Pharmacol. 2015 Sep;88(3):450-9
pubmed: 26089372
Pharmacol Ther. 2011 Apr;130(1):9-25
pubmed: 21256155
Front Physiol. 2016 Sep 30;7:441
pubmed: 27746744
Neuropharmacology. 2018 Sep 15;140:275-286
pubmed: 30102917
J Biol Chem. 2014 Feb 21;289(8):5296-309
pubmed: 24394414
Nat Protoc. 2018 May;13(5):1062-1090
pubmed: 29674755
Cell Chem Biol. 2017 Mar 16;24(3):360-370
pubmed: 28286129
JCI Insight. 2019 Apr 18;4(8):
pubmed: 30996138
Proc Natl Acad Sci U S A. 2009 Jun 9;106(23):9501-6
pubmed: 19470481
Biopolymers. 2005;80(2-3):357-66
pubmed: 15810013
Neuron. 1999 Jul;23(3):487-98
pubmed: 10433261
Pharmacol Rev. 2019 Oct;71(4):571-595
pubmed: 31551350
Nature. 2015 Jan 15;517(7534):302-10
pubmed: 25592535
J Clin Endocrinol Metab. 1983 Mar;56(3):549-52
pubmed: 6822652
PLoS One. 2018 Apr 3;13(4):e0195316
pubmed: 29614098
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):4040-5
pubmed: 9520489
J Biol Chem. 2005 Nov 11;280(45):37917-23
pubmed: 16147994
Mol Pharmacol. 2015 Nov;88(5):853-65
pubmed: 26290606
J Pharmacol Exp Ther. 2004 Feb;308(2):627-35
pubmed: 14593085
J Neurosci. 2013 Sep 18;33(38):15235-47
pubmed: 24048853
Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4814-9
pubmed: 10781086
Nat Commun. 2019 Oct 16;10(1):4693
pubmed: 31619668
Elife. 2016 Jul 19;5:
pubmed: 27434672
Nat Rev Drug Discov. 2017 Dec;16(12):829-842
pubmed: 29075003
J Clin Invest. 2000 Jun;105(11):1595-604
pubmed: 10841518
FEBS Lett. 1999 Apr 1;448(1):180-4
pubmed: 10217436
Nature. 2019 Feb;566(7742):79-84
pubmed: 30675062
Sci Rep. 2012;2:496
pubmed: 22773945
Nephron Clin Pract. 2006;103(3):c106-13
pubmed: 16534234
J Biol Chem. 2002 Sep 13;277(37):33727-35
pubmed: 12095982
Physiol Rev. 2001 Jan;81(1):239-297
pubmed: 11152759
Sci Adv. 2016 May 27;2(5):e1600241
pubmed: 27386547
Acta Crystallogr D Biol Crystallogr. 2014 Apr;70(Pt 4):1104-14
pubmed: 24699654

Auteurs

Hongkang Liu (H)

Cellular Signaling Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
International Research Center for Sensory Biology and Technology of Ministry of Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, 34094 Montpellier Cedex 5, France.

Ping Yi (P)

Cellular Signaling Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
International Research Center for Sensory Biology and Technology of Ministry of Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.

Wenjing Zhao (W)

Cellular Signaling Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
International Research Center for Sensory Biology and Technology of Ministry of Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.

Yuling Wu (Y)

Cellular Signaling Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
International Research Center for Sensory Biology and Technology of Ministry of Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.

Francine Acher (F)

Faculté des Sciences Fondamentales et Biomédicales, CNRS, Université de Paris, 75270 Paris Cedex 6, France.

Jean-Philippe Pin (JP)

Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, 34094 Montpellier Cedex 5, France; jean-philippe.pin@igf.cnrs.fr jfliu@mail.hust.edu.cn.

Jianfeng Liu (J)

Cellular Signaling Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China; jean-philippe.pin@igf.cnrs.fr jfliu@mail.hust.edu.cn.
International Research Center for Sensory Biology and Technology of Ministry of Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, 430074 Wuhan, China.
Guangzhou Regenerative Medicine and Health Guangdong Laboratory, Chinese Academy of Sciences, 510005 Guangzhou, China.

Philippe Rondard (P)

Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, 34094 Montpellier Cedex 5, France.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH