Microtubules Enhance Mesoscale Effective Diffusivity in the Crowded Metaphase Cytoplasm.
cell division
cytoplasm
diffusion
macromolecular crowding
metaphase
microtubules
mitotic spindle
nanoparticles
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
14 09 2020
14 09 2020
Historique:
received:
29
01
2020
revised:
10
06
2020
accepted:
24
07
2020
pubmed:
21
8
2020
medline:
24
2
2021
entrez:
21
8
2020
Statut:
ppublish
Résumé
Mesoscale macromolecular complexes and organelles, tens to hundreds of nanometers in size, crowd the eukaryotic cytoplasm. It is therefore unclear how mesoscale particles remain sufficiently mobile to regulate dynamic processes such as cell division. Here, we study mobility across dividing cells that contain densely packed, dynamic microtubules, comprising the metaphase spindle. In dividing human cells, we tracked 40 nm genetically encoded multimeric nanoparticles (GEMs), whose sizes are commensurate with the inter-filament spacing in metaphase spindles. Unexpectedly, the effective diffusivity of GEMs was similar inside the dense metaphase spindle and the surrounding cytoplasm. Eliminating microtubules or perturbing their polymerization dynamics decreased diffusivity by ~30%, suggesting that microtubule polymerization enhances random displacements to amplify diffusive-like motion. Our results suggest that microtubules effectively fluidize the mitotic cytoplasm to equalize mesoscale mobility across a densely packed, dynamic, non-uniform environment, thus spatially maintaining a key biophysical parameter that impacts biochemistry, ranging from metabolism to the nucleation of cytoskeletal filaments.
Identifiants
pubmed: 32818469
pii: S1534-5807(20)30595-5
doi: 10.1016/j.devcel.2020.07.020
pmc: PMC7685229
mid: NIHMS1623241
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
574-582.e4Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM132447
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130234
Pays : United States
Organisme : NCI NIH HHS
ID : R37 CA240765
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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