The possible effect of silver nanoparticles on glyceraldehyde-3-phosphate dehydrogenase activity and formation of amyloid-like aggregates in MCF-7 cell line.
Amyloid
/ drug effects
Animals
Apoptosis
/ drug effects
Cattle
Cell Survival
/ drug effects
Gene Expression Regulation, Enzymologic
/ drug effects
Glyceraldehyde-3-Phosphate Dehydrogenases
/ genetics
Humans
MCF-7 Cells
Metal Nanoparticles
/ chemistry
Oxidative Stress
/ drug effects
Reactive Oxygen Species
/ metabolism
Silver
/ pharmacology
amyloid aggregates
apoptosis
glyceraldehydes-3-phosphate dehydrogenase
oxidative stress
silver nanoparticles
Journal
IUBMB life
ISSN: 1521-6551
Titre abrégé: IUBMB Life
Pays: England
ID NLM: 100888706
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
28
05
2020
revised:
02
07
2020
accepted:
08
07
2020
pubmed:
21
8
2020
medline:
15
12
2021
entrez:
21
8
2020
Statut:
ppublish
Résumé
Silver nanoparticles (AgNPs) are widely used in medicine, however, the underlying mechanisms of their action on cellular signaling have not been completely determined, and fundamental studies are required to clarify them. We aimed to investigate AgNPs effects on glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as both the internal control gene and the redox-sensitive enzyme involved in apoptosis-related pathways and the formation of amyloid aggregates. To achieve this purpose, MCF-7 cells were treated with different concentrations (0, 3, 22, and 200 μg/ml) of AgNPs and then cell viability, generation of reactive oxygen species (ROS), induction of apoptosis, expression of GAPDH gene, the formation of amyloid aggregates, and GAPDH activity were assessed. The results indicated that treatment with AgNPs significantly reduced cell viability and increased apoptosis in a dose-dependent manner. The ROS levels increased at lower concentrations of AgNPs (up to 22 μg/ml) and during short-term exposure (30 min). The level of GAPDH gene expression was significantly upregulated by 1.22, 1.47, and 1.56 fold, in the concentrations of 3, 22, and 200 μg/ml, respectively. The amount of amyloid aggregates was significantly increased in a dose-dependent manner. The results of enzyme activity showed that AgNPs were affected on the activity of GAPDH protein, however, it has fluctuated that could not be interpreted by our limited data. In conclusion, our results suggested that AgNPs could affect the GAPDH gene expression and enzyme activity, therefore the selection of GAPDH as a gene and protein internal control in the (AgNPs)-related studies requires careful consideration. Additionally, AgNPs may cause apoptosis due to the increase in the production of amyloid aggregates.
Substances chimiques
Amyloid
0
Reactive Oxygen Species
0
Silver
3M4G523W1G
Glyceraldehyde-3-Phosphate Dehydrogenases
EC 1.2.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2214-2224Informations de copyright
© 2020 International Union of Biochemistry and Molecular Biology.
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