Dysbiosis in intestinal microbiome linked to fecal blood determined by direct hybridization.
Clostridium difficile
Direct detection
Dysbiosis
Eubiosis
Microbiome
Proteobacteria
Journal
3 Biotech
ISSN: 2190-572X
Titre abrégé: 3 Biotech
Pays: Germany
ID NLM: 101565857
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
23
03
2020
accepted:
21
07
2020
entrez:
22
8
2020
pubmed:
22
8
2020
medline:
22
8
2020
Statut:
ppublish
Résumé
The important physiological and pathophysiological roles of intestinal human microbiome (HMB) in human health have been emerging, owing to the access to molecular biology techniques. Herein we evaluated, for the first time, the intestinal HMB through direct hybridization approach using n-counter flex DX technology which bypasses the amplification procedure currently applied by other technologies to study the human microbiome. To this purpose, a clinical study was carried out on fecal samples, recruiting both healthy volunteers (N-FOB) and subjects positive for occult blood (P-FOB). A relevant custom panel of 79 16S rRNA target gene was engineered and 32 of them displayed a variation between the two clusters of subjects. Our findings revealed that bacteria belonging to Proteobacteria have higher distribution in P-FOB describing dysbiosis. Similarly, Bacteroidetes and Firmicutes phylum display high distribution in P-FOB. Of interest, the presence of
Identifiants
pubmed: 32821643
doi: 10.1007/s13205-020-02351-w
pii: 2351
pmc: PMC7387388
doi:
Types de publication
Case Reports
Langues
eng
Pagination
358Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Conflict of interestC.C., T.M. and E.C. are co-inventors of an Italian patent deposit held by Artemisia Lab Srl, and GalaScreen Srl (Ref. Number: 102018000011030 12/12/2018).
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