Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha.
CD8
canine
immunohistochemistry
monoclonal antibody
Journal
Monoclonal antibodies in immunodiagnosis and immunotherapy
ISSN: 2167-9436
Titre abrégé: Monoclon Antib Immunodiagn Immunother
Pays: United States
ID NLM: 101590955
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
entrez:
22
8
2020
pubmed:
22
8
2020
medline:
25
6
2021
Statut:
ppublish
Résumé
Understanding of the microenvironment of cancer plays a crucial role in cancer research. A tool is needed to evaluate the immune cells surrounding the cancer cells. This study establishes and evaluates a novel monoclonal antibody against canine CD8α (cCD8α). The antibody was produced by immunization of rats with cCD8α-expressing cells. After establishment and selection of hybridoma cells, the clone F3-B2 was established. The reactivity of F3-B2 was confirmed using cCD8α-overexpressing murine cells. Flow cytometric analysis also demonstrated that F3-B2 reacts with cCD8α naturally expressed in canine peripheral blood mononuclear cells and a canine T cell lymphoma cell line. The specimens of lymphoid tissue showed immunohistochemical staining for F3-B2. Moreover, we also found that F3-B2 exhibited reactivity against feline CD8. Thus, this antibody provides a good research tool to analyze CD8-positive cytotoxic lymphocytes in canine and feline tumors.
Identifiants
pubmed: 32822285
doi: 10.1089/mab.2020.0002
doi:
Substances chimiques
Antibodies, Monoclonal
0
CD8 Antigens
0
CD8 antigen, alpha chain
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM