Genotoxic effect of 2,2'-bis(bicyclo[2.2.1] heptane) on bacterial cells.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
16
01
2020
accepted:
07
08
2020
entrez:
22
8
2020
pubmed:
22
8
2020
medline:
2
10
2020
Statut:
epublish
Résumé
The toxic effect of strained hydrocarbon 2,2'-bis (bicyclo[2.2.1]heptane) (BBH) was studied using whole-cell bacterial lux-biosensors based on Escherichia coli cells in which luciferase genes are transcriptionally fused with stress-inducible promoters. It was shown that BBH has the genotoxic effect causing bacterial SOS response however no alkylating effect has been revealed. In addition to DNA damage, there is an oxidative effect causing the response of OxyR/S and SoxR/S regulons. The most sensitive to BBH lux-biosensor was E. coli pSoxS-lux which reacts to the appearance of superoxide anion radicals in the cell. It is assumed that the oxidation of BBH leads to the generation of reactive oxygen species, which provide the main contribution to the genotoxicity of this substance.
Identifiants
pubmed: 32822344
doi: 10.1371/journal.pone.0228525
pii: PONE-D-20-01518
pmc: PMC7444485
doi:
Substances chimiques
Bridged Bicyclo Compounds
0
Mutagens
0
bicyclo(2.2.1)heptene
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0228525Commentaires et corrections
Type : ErratumIn
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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