MiRNA let-7 from TPO(+) Extracellular Vesicles is a Potential Marker for a Differential Diagnosis of Follicular Thyroid Nodules.
Adenocarcinoma, Follicular
/ blood
Adenoma
/ blood
Autoantigens
/ metabolism
Biomarkers, Tumor
/ blood
Case-Control Studies
Diagnosis, Differential
Extracellular Vesicles
/ metabolism
Female
Humans
Iodide Peroxidase
/ metabolism
Iron-Binding Proteins
/ metabolism
Male
MicroRNAs
/ genetics
Middle Aged
Real-Time Polymerase Chain Reaction
Thyroid Nodule
/ blood
EV
Let-7
TPO
biomarker
follicular adenoma
liquid biopsy
miRNA
thyroid cancer
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
18 08 2020
18 08 2020
Historique:
received:
19
07
2020
revised:
14
08
2020
accepted:
15
08
2020
entrez:
23
8
2020
pubmed:
23
8
2020
medline:
20
3
2021
Statut:
epublish
Résumé
The current approaches to distinguish follicular adenomas (FA) and follicular thyroid cancer (FTC) at the pre-operative stage have low predictive value. Liquid biopsy-based analysis of circulating extracellular vesicles (EVs) presents a promising diagnostic method. However, the extreme heterogeneity of plasma EV population hampers the development of new diagnostic tests. We hypothesize that the isolation of EVs with thyroid-specific surface molecules followed by miRNA analysis, may have improved diagnostic potency. The total population of EVs was isolated from the plasma of patients with FA ( TPO(+)EVs can be efficiently isolated by immunobeads. The analysis of Let-7 family members in TPO(+)EVs allows one to distinguish FA and FTC with high accuracy (area under curve defined by ROC = 0.77-0.84). The isolation of TPO(+)EVs, followed by RT-qPCR analysis of Let-7 family members, may present a helpful approach to manage follicular nodules in the thyroid gland.
Sections du résumé
BACKGROUND
The current approaches to distinguish follicular adenomas (FA) and follicular thyroid cancer (FTC) at the pre-operative stage have low predictive value. Liquid biopsy-based analysis of circulating extracellular vesicles (EVs) presents a promising diagnostic method. However, the extreme heterogeneity of plasma EV population hampers the development of new diagnostic tests. We hypothesize that the isolation of EVs with thyroid-specific surface molecules followed by miRNA analysis, may have improved diagnostic potency.
METHODS
The total population of EVs was isolated from the plasma of patients with FA (
RESULTS
TPO(+)EVs can be efficiently isolated by immunobeads. The analysis of Let-7 family members in TPO(+)EVs allows one to distinguish FA and FTC with high accuracy (area under curve defined by ROC = 0.77-0.84).
CONCLUSION
The isolation of TPO(+)EVs, followed by RT-qPCR analysis of Let-7 family members, may present a helpful approach to manage follicular nodules in the thyroid gland.
Identifiants
pubmed: 32824820
pii: cells9081917
doi: 10.3390/cells9081917
pmc: PMC7465637
pii:
doi:
Substances chimiques
Autoantigens
0
Biomarkers, Tumor
0
Iron-Binding Proteins
0
MicroRNAs
0
mirnlet7 microRNA, human
0
TPO protein, human
EC 1.11.1.7
Iodide Peroxidase
EC 1.11.1.8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Eur Thyroid J. 2017 Jul;6(4):171-177
pubmed: 28868257
HNO. 2020 Feb;68(2):100-105
pubmed: 32006045
Tumour Biol. 2016 Sep;37(9):12011-12021
pubmed: 27164936
Curr Drug Targets. 2018;19(14):1721-1729
pubmed: 29484992
Transl Oncol. 2009 Dec;2(4):236-41
pubmed: 19956384
Int J Hematol Oncol Stem Cell Res. 2018 Jul 1;12(3):235-248
pubmed: 30595827
Front Endocrinol (Lausanne). 2019 Nov 12;10:783
pubmed: 31781047
Clin Cancer Res. 2019 Jan 1;25(1):266-276
pubmed: 30287549
Thyroid. 2018 Oct;28(10):1229-1242
pubmed: 30039751
Clin Endocrinol (Oxf). 2018 Jun;88(6):936-942
pubmed: 29509975
Thyroid. 2017 Nov;27(11):1341-1346
pubmed: 29091573
Diagnostics (Basel). 2020 Feb 28;10(3):
pubmed: 32121086
Int J Mol Sci. 2019 Mar 05;20(5):
pubmed: 30841521
J Thyroid Res. 2016;2016:9276402
pubmed: 27379194
J Clin Endocrinol Metab. 2019 Oct 1;104(10):4607-4618
pubmed: 31199456
J Thyroid Res. 2011;2011:631593
pubmed: 22496986
J Extracell Vesicles. 2014 Dec 22;3:26913
pubmed: 25536934
Mol Cell Endocrinol. 2020 Apr 15;506:110756
pubmed: 32045626
Nat Methods. 2017 Feb 28;14(3):228-232
pubmed: 28245209
Int J Mol Cell Med. 2019 Winter;8(Suppl1):19-29
pubmed: 32351906
Biomark Insights. 2008 Apr 29;3:237-252
pubmed: 19578508
Thromb Res. 2010 Apr;125 Suppl 1:S49-51
pubmed: 20149923
Contemp Oncol (Pozn). 2018 Mar;22(1A):38-40
pubmed: 29628792
Int J Endocrinol. 2020 Apr 28;2020:4378345
pubmed: 32411222
PLoS One. 2018 Mar 7;13(3):e0193624
pubmed: 29513734
Gland Surg. 2018 Aug;7(Suppl 1):S19-S29
pubmed: 30175060
Radiol Bras. 2019 Jul-Aug;52(4):247-253
pubmed: 31435087
J Extracell Vesicles. 2018 Nov 23;7(1):1535750
pubmed: 30637094
Oncoimmunology. 2020 Apr 7;9(1):1747732
pubmed: 32313730
Gene. 2019 Apr 20;693:37-45
pubmed: 30684524