Impact of Extracellular Vesicle Isolation Methods on Downstream Mirna Analysis in Semen: A Comparative Study.
biomarker
diagnosis
exosomes
extracellular vesicle isolation methods
extracellular vesicle miRNA analysis
extracellular vesicles
microvesicles
prostate cancer
semen
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
19 Aug 2020
19 Aug 2020
Historique:
received:
30
07
2020
revised:
14
08
2020
accepted:
15
08
2020
entrez:
23
8
2020
pubmed:
23
8
2020
medline:
17
2
2021
Statut:
epublish
Résumé
Seminal plasma (SP) contains a unique concentration of miRNA, mostly contained in small extracellular vesicles (sEVs) such as exosomes, some of which could be clinically useful for diagnosis and/or prognosis of urogenital diseases such as prostate cancer (PCa). We optimized several exosome-EV isolation technologies for their use in semen, evaluating EV purifying effectiveness and impact on the downstream analysis of miRNAs against results from the standard ultracentrifugation (UC) method to implement the use of SP sEV_miRNAs as noninvasive biomarkers for PCa. Our results evidenced that commercial kits designed to isolate exosomes/EVs from blood or urine are mostly applicable to SP, but showed quantitative and qualitative variability between them.
Identifiants
pubmed: 32824915
pii: ijms21175949
doi: 10.3390/ijms21175949
pmc: PMC7504614
pii:
doi:
Substances chimiques
Biomarkers, Tumor
0
MicroRNAs
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Instituto de Salud Carlos III
ID : PI15/00153; DTS18/00101
Organisme : Generalitat de Catalunya
ID : 2017SGR191
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