Towards a Sampling Rationale for African Swine Fever Virus Detection in Pork Products.
African swine fever virus
food testing
qPCR
sausage
surveillance
Journal
Foods (Basel, Switzerland)
ISSN: 2304-8158
Titre abrégé: Foods
Pays: Switzerland
ID NLM: 101670569
Informations de publication
Date de publication:
20 Aug 2020
20 Aug 2020
Historique:
received:
17
07
2020
revised:
11
08
2020
accepted:
18
08
2020
entrez:
23
8
2020
pubmed:
23
8
2020
medline:
23
8
2020
Statut:
epublish
Résumé
African swine fever (ASF) is a highly lethal disease of pigs caused by the ASF virus (ASFV), which presents a serious threat to global food security. The movement of contaminated pork products has previously been postulated as contributing to the introduction of ASF into new areas. To evaluate the performance of ASFV detection systems in multi-component pork products, we spiked sausage meat with four different ASFV-containing materials (ASFV cell culture, pork loin, meat juice and bone marrow). DNA was extracted using two manual systems (MagMAX CORE, Qiagen) and one automated (MagMAX CORE) one, and three qPCR assays (VetMAX, King, UPL) were used. The performance of the DNA extraction systems was as follows; automated MagMAX > manual MagMAX > manual Qiagen. The commercial VetMAX qPCR assay yielded significantly lower C
Identifiants
pubmed: 32825271
pii: foods9091148
doi: 10.3390/foods9091148
pmc: PMC7554881
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007036
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007037
Pays : United Kingdom
Organisme : Department for Environment, Food and Rural Affairs, UK Government
ID : SE26081
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007036 and BBS/E/I/00007037
Pays : United Kingdom
Références
Prev Vet Med. 2019 Jan 1;162:126-130
pubmed: 30621891
Vet Rec. 2019 Aug 3;185(5):124-125
pubmed: 31371673
Viruses. 2019 Mar 30;11(4):
pubmed: 30935026
Emerg Microbes Infect. 2019;8(1):1000-1002
pubmed: 31267844
Vaccines (Basel). 2017 Oct 07;5(4):
pubmed: 28991171
J Clin Microbiol. 2015 Aug;53(8):2555-65
pubmed: 26041901
J Vet Res. 2019 Sep 13;63(3):303-310
pubmed: 31572808
Emerg Infect Dis. 2019 Jun;25(6):1231-1233
pubmed: 30844357
Transbound Emerg Dis. 2013 Feb;60(1):48-58
pubmed: 22394449
J Virol Methods. 2003 Jan;107(1):53-61
pubmed: 12445938
J Virol. 2020 Mar 17;94(7):
pubmed: 31969432
J Comp Pathol. 2015 Jan;152(1):9-21
pubmed: 25443146
BMC Vet Res. 2019 Feb 22;15(1):64
pubmed: 30795759
Transbound Emerg Dis. 2020 May 18;:
pubmed: 32419367
Vet J. 2018 Mar;233:41-48
pubmed: 29486878
J Virol Methods. 2010 Mar;164(1-2):68-74
pubmed: 19963011
EFSA J. 2017 Nov 07;15(11):e05068
pubmed: 32625356
Vaccines (Basel). 2019 Jun 25;7(2):
pubmed: 31242632
Virus Res. 2013 Apr;173(1):198-203
pubmed: 23266725
Emerg Infect Dis. 2008 Dec;14(12):1870-4
pubmed: 19046509
Vet Rec. 2016 Mar 12;178(11):262-7
pubmed: 26966305
Front Vet Sci. 2020 Jan 09;6:486
pubmed: 31998765
Science. 2020 Feb 7;367(6478):622-624
pubmed: 32029612
Transbound Emerg Dis. 2019 Jan;66(1):54-55
pubmed: 30383329