Bacteroides fragilis fucosidases facilitate growth and invasion of Campylobacter jejuni in the presence of mucins.
Bacteroides fragillis
Campylobacter jejuni
fucosidase
l-fucose
mucin
virulence
Journal
Cellular microbiology
ISSN: 1462-5822
Titre abrégé: Cell Microbiol
Pays: India
ID NLM: 100883691
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
16
04
2020
revised:
23
07
2020
accepted:
24
07
2020
pubmed:
23
8
2020
medline:
7
8
2021
entrez:
23
8
2020
Statut:
ppublish
Résumé
The enteropathogenic bacterium, Campylobacter jejuni, was considered to be non-saccharolytic, but recently it emerged that l-fucose plays a central role in C. jejuni virulence. Half of C. jejuni clinical isolates possess an operon for l-fucose utilisation. In the intestinal tract, l-fucose is abundantly available in mucin O-linked glycan structures, but C. jejuni lacks a fucosidase enzyme essential to release the l-fucose. We set out to determine how C. jejuni can gain access to these intestinal l-fucosides. Growth of the fuc + C. jejuni strains, 129,108 and NCTC 11168, increased in the presence of l-fucose while fucose permease knockout strains did not benefit from additional l-fucose. With fucosidase assays and an activity-based probe, we confirmed that Bacteriodes fragilis, an abundant member of the intestinal microbiota, secretes active fucosidases. In the presence of mucins, C. jejuni was dependent on B. fragilis fucosidase activity for increased growth. Campylobacter jejuni invaded Caco-2 intestinal cells that express complex O-linked glycan structures that contain l-fucose. In infection experiments, C. jejuni was more invasive in the presence of B. fragilis and this increase is due to fucosidase activity. We conclude that C. jejuni fuc + strains are dependent on exogenous fucosidases for increased growth and invasion.
Identifiants
pubmed: 32827216
doi: 10.1111/cmi.13252
pmc: PMC7685106
doi:
Substances chimiques
Mucins
0
Fucose
28RYY2IV3F
alpha-L-Fucosidase
EC 3.2.1.51
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13252Informations de copyright
© 2020 The Authors. Cellular Microbiology published by John Wiley & Sons Ltd.
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