Detecting Mechanical Anisotropy of the Cornea Using Brillouin Microscopy.
Brillouin microscopy
corneal anisotropy
corneal cross-linking
keratoconus
Journal
Translational vision science & technology
ISSN: 2164-2591
Titre abrégé: Transl Vis Sci Technol
Pays: United States
ID NLM: 101595919
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
25
02
2020
accepted:
28
04
2020
entrez:
25
8
2020
pubmed:
25
8
2020
medline:
25
8
2020
Statut:
epublish
Résumé
The purpose of this study was to detect the mechanical anisotropy of the cornea using Brillouin microscopy along different perturbation directions. Brillouin frequency shift of both whole globes ( We detected mechanical anisotropy of corneas, with an average frequency shift increase of 53 MHz and 96 MHz when the instrument probed from 0° to 15° and 30° along the direction of the stromal fibers. Brillouin microscopy did not lose sensitivity to mechanical anisotropy up to 96% water content. We experimentally measured and theoretically modeled how mechanical changes independent of hydration affect frequency shift as a result of corneal cross-linking by isolating an approximately 100 MHz increase in frequency shift following a cross-linking procedure purely due to changes of stromal tissue mechanics. Brillouin microscopy is sensitive to mechanical anisotropy of the stroma even in highly hydrated corneas. The agreement between model and experimental data suggested a quantitative relationship between Brillouin frequency shift, hydration state of the cornea, and stromal tissue stiffness. The protocol and model validated throughout this study offer a path for comprehensive measurements of corneal mechanics within the clinic; allowing for improved evaluation of the long-term mechanical efficacy of cross-linking procedures.
Identifiants
pubmed: 32832232
doi: 10.1167/tvst.9.7.26
pii: TVST-20-2367
pmc: PMC7414627
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Pagination
26Subventions
Organisme : NEI NIH HHS
ID : R01 EY028666
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD095520
Pays : United States
Informations de copyright
Copyright 2020 The Authors.
Déclaration de conflit d'intérêts
Disclosure: J.N. Webb, None; H. Zhang, None; A. Sinha Roy, None; J.B. Randleman, None; G. Scarcelli, (P) related to Brillouin technology
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