The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus.
plaque assay
propagation
quantification
stock
storage
titration
vesicular stomatitis virus
Journal
Current protocols in microbiology
ISSN: 1934-8533
Titre abrégé: Curr Protoc Microbiol
Pays: United States
ID NLM: 101257113
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
entrez:
25
8
2020
pubmed:
25
8
2020
medline:
26
5
2021
Statut:
ppublish
Résumé
Vesicular stomatitis virus (VSV) is the prototypical member of the Rhabdoviridae family of negative-sense single-stranded RNA viruses. This virus has been used as a powerful model system for decades and is currently being used as a vaccine platform and an oncolytic agent. Here, we present methods to propagate, quantitate, and store VSV. We also review the proper safety protocol for the handling of VSV, which is classified as a Biosafety Level 2 pathogen by the United States Centers for Disease Control and Prevention. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation, purification, and storage of vesicular stomatitis virus stocks Basic Protocol 2: Quantification of vesicular stomatitis virus by plaque assay Support Protocol: Propagation of Vero cells.
Identifiants
pubmed: 32833351
doi: 10.1002/cpmc.110
pmc: PMC7449588
mid: NIHMS1617011
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e110Subventions
Organisme : NIAID NIH HHS
ID : R15 AI058969
Pays : United States
Informations de copyright
© 2020 Wiley Periodicals LLC.
Références
J Gen Virol. 2012 Dec;93(Pt 12):2529-2545
pubmed: 23052398
Proc Natl Acad Sci U S A. 1995 Aug 29;92(18):8388-92
pubmed: 7667300
Int J Clin Exp Med. 2014 May 15;7(5):1204-13
pubmed: 24995075
J Virol. 2002 Jan;76(2):895-904
pubmed: 11752178
Front Oncol. 2020 Apr 16;10:475
pubmed: 32373515
J Virol. 2013 Sep;87(18):10283-94
pubmed: 23864625
Curr Protoc Microbiol. 2016 Nov 18;43:15D.4.1-15D.4.16
pubmed: 27858969
Vaccine X. 2019 Jan 29;1:100009
pubmed: 31384731
Sci Transl Med. 2015 Dec 9;7(317):317ps24
pubmed: 26659569
Rev Med Virol. 2018 Nov;28(6):e2008
pubmed: 30209859
N Engl J Med. 2017 Jan 26;376(4):330-341
pubmed: 25830322
Hum Vaccin Immunother. 2018;14(9):2107-2113
pubmed: 29757706
MethodsX. 2020 Feb 20;7:100806
pubmed: 32195130
J Vis Exp. 2014 Nov 04;(93):e52065
pubmed: 25407402
J Virol. 2010 Apr;84(7):3280-6
pubmed: 20032173
J Comp Neurol. 2015 Aug 1;523(11):1639-63
pubmed: 25688551