Human L-Dopa decarboxylase interaction with annexin V and expression during apoptosis.
Animals
Annexin A5
/ metabolism
Apoptosis
/ drug effects
Aromatic-L-Amino-Acid Decarboxylases
/ genetics
Cell Death
/ drug effects
Cell Line
Cell Line, Tumor
Cobalt
/ toxicity
Cricetinae
Enzyme Inhibitors
/ metabolism
Female
Humans
Placenta
/ metabolism
Pregnancy
Protein Isoforms
/ genetics
Staurosporine
/ toxicity
Alternative l-Dopa decarboxylase (alt-DDC)
Annexin V
Apoptosis
Cytotoxicity
L-dopa decarboxylase
Staurosporine
Journal
Biochimie
ISSN: 1638-6183
Titre abrégé: Biochimie
Pays: France
ID NLM: 1264604
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
06
06
2020
revised:
30
07
2020
accepted:
17
08
2020
pubmed:
25
8
2020
medline:
28
5
2021
entrez:
25
8
2020
Statut:
ppublish
Résumé
l-Dopa Decarboxylase (DDC) is a pyridoxal requiring enzyme that catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (l-Dopa) to Dopamine (DA). The function of DDC in physiological and pathological biochemical pathways remains poorly understood, while the function and regulation of human DDC isoforms is almost completely elusive. We have shown that Annexin V, a fundamental apoptosis marker, is an inhibitor of l-Dopa decarboxylase activity. Here we show the interaction of both the full-length DDC and the truncated isoform alternative DDC (Alt-DDC) with Annexin V in human tissue and cell lines. Interestingly, DDC isoform expression is enhanced or remains unaffected following staurosporine (STS) treatment, despite increased levels of cytotoxicity and apoptosis. The findings presented here provide novel insights concerning the involvement of DDC in programmed cell death.
Identifiants
pubmed: 32835737
pii: S0300-9084(20)30194-2
doi: 10.1016/j.biochi.2020.08.010
pii:
doi:
Substances chimiques
Annexin A5
0
Enzyme Inhibitors
0
Protein Isoforms
0
Cobalt
3G0H8C9362
Aromatic-L-Amino-Acid Decarboxylases
EC 4.1.1.28
DDC protein, human
EC 4.1.1.28
cobaltous chloride
EVS87XF13W
Staurosporine
H88EPA0A3N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
78-86Informations de copyright
Copyright © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflict of interest.